First synthetic analogues of diphosphoinositol polyphosphates: interaction with PP-InsP(5) kinase

• Published in: Chemical communications (Cambridge, England) Vol. 48; no. 92; pp. 11292 - 11294
• Main Authors: Riley, Andrew M., Wang, Huanchen, Weaver, Jeremy D., Shears, Stephen B., Potter, Barry V. L.
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 30.07.2013
• Subjects: Chemistry; Chemistry, Multidisciplinary; Physical Sciences; Science & Technology; DICTYOSTELIUM; CELLS; PENTAKISPHOSPHATE; MEDIATORS; BINDING; INOSITOL; PYROPHOSPHATES

We synthesised analogues of diphosphoinositol polyphosphates (PP-InsPs) in which the diphosphate is replaced by an a-phosphonoacetic acid (PA) ester. Structural analysis revealed that 5-PA-InsP(5) mimics 5-PP-InsP(5) binding to the kinase domain of PPIP5K2; both molecules were phosphorylated by the...