Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array

This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybr...

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Published inDenki kagaku oyobi kōgyō butsuri kagaku Vol. 84; no. 5; p. 338
Main Authors OHARA, Masayuki, SEKIYA, Yusuke, KAWANO, Ryuji
Format Journal Article
LanguageJapanese
Published 01.01.2016
Subjects
Arrays
Biological
Computer simulation
Correlation
Deoxyribonucleic acid
Mathematical models
Nanostructure
Replication
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Abstract This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybridization energy of the duplexes was able to be modeled as first-ordered reaction. This method is one of the approach for understanding of basic biological reactions in DNA replication and RNA transcription.
AbstractList This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybridization energy of the duplexes was able to be modeled as first-ordered reaction. This method is one of the approach for understanding of basic biological reactions in DNA replication and RNA transcription.
Author KAWANO, Ryuji
SEKIYA, Yusuke
OHARA, Masayuki
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Snippet This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and...
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StartPage 338
SubjectTerms Arrays
Biological
Computer simulation
Correlation
Deoxyribonucleic acid
Mathematical models
Nanostructure
Replication
Title Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array
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