夏枯草의 간세포에서 항산화 및 미토콘드리아 보호효과

• Published in: 동의생리병리학회지 Vol. 30; no. 1; pp. 20 - 26
• Main Authors: 장미희(Mihee Jang), 서혜림(Hye Lim Seo), 김상찬(Sang Chan Kim), 김영우(Young Woo Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.02.2016
• Subjects: 한의학; Prunellae Spica; AMPK; Mitochondria; Oxidative Stress; Liver
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2016.02.30.1.20

Prunellae Spica, the herbaceous plant in the genus Prunella, is a traditional herbal medicine and has been reported to have diuretic, anti-bacterial and anti-oxidant effects. However, the mechanism of its action was not clearly identified. In the present study, we investigated the hepatoprotective effect of Prunellae Spica extract (PSE) against the damage of mitochondria and death in hepatocyte induced by oxidative stress. Treatment of arachidonic acid (AA)+iron significantly induced oxidative stress and apoptosis in the hepatocytes. However, PSE protected cells and inhibited apoptosis by altering the protein levels such as poly(ADP-ribose) polymerase and pro-caspase 3. Moreover, AA+iron induced reactive oxygen species production and mitochondrial dysfunction, and Both of them were inhibited by PSE treatment. PSE markedly activated AMP-activated protein kinase (AMPK), an important regulator in cell survival. Furthermore, this activation by PSE was mediated with liver kinase B1, a major upstream kinase that phosphorylates Thr 172 of AMPKα, and this activation was associated with its cell protection, as assessed by an experiment of a chemical inhibitor. In conclusion, this study demonstrate that PSE protects hepatocytes against oxidative stress as mediated with activation of LKB1-dependent AMPK pathway. KCI Citation Count: 0