인체 위암세포주에서 半枝蓮의 항암 효능에 관한 연구

• Published in: 동의생리병리학회지 Vol. 29; no. 2; pp. 195 - 201
• Main Authors: 심지환(Ji Hwan Shim), 이수진(Soojin Lee), 김희진(Huijin Gim), 박현수(Hyun Soo Park), 김병주(Byung Joo Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.04.2015
• Subjects: 한의학; Cutellaria barbata; Human gastric adenocarcinoma cancer cell; AGS; Anti-cancer drugs
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2015.04.29.2.195

The aim of the study is to investigate the anti-cancer effects of Scutellaria barbata in AGS human gastric adenocarcinoma cells. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and caspase 3 or 9 activity assay were carried out to examine cell death with Scutellaria barbata. To elucidate the inhibitory effects of Scutellaria barbata, cell cycle (sub-G1) analysis and mitochondrial membrane potential were performed in AGS cells after 24 h incubation with Scutellaria barbata. Scutellaria barbata induced apoptosis in AGS cells by using the MTT assay, the sub-G1 analysis and mitochondrial membrane potential assay. The stronger inhibition effects of AGS cell growth was observed by application of Scutellaria barbata combined with several anti-cancer drugs (paclitaxel, 5-fluorouracil, cisplatin, ectoposide, doxorubicin and docetaxel) in comparison to the application of Scutellaria barbata or anti-cancer drugs. Our findings provide insight into unraveling the effects of Scutellaria barbata in human gastric cancer cells and developing therapeutic agents against gastric cancer. KCI Citation Count: 1