복벽 손상 백서에서 세가지 유형의 유착방지제의 효과

• Published in: Annals of surgical treatment and research Vol. 77; no. 1; pp. 7 - 14
• Main Authors: 송형준(Hyung Jun Song), 김종원(Jong Won Kim), 박준석(Jun Seok Park), 김용석(Yong Seok Kim), 최유신(Yoo Shin Choi), 김범규(Beom Gyu Kim), 차성재(Sung Jae Cha), 박성준(Sung Jun Park), 장인택(In Taik Chang), 박성일(Sung Il Park), 박언섭(Eon Sub Park), 홍순억(Soon Auck Hong)
• Format: Journal Article
• Language: Korean
• Published: 대한외과학회 01.07.2009
• Subjects: 일반외과학; Animal model; 유착; 큰포식세포; 미세혈관밀도; Antiadhesive agents; Adhesion; 유착방지제; Microvessel density(MVD); Macrophage; 동물실험
• ISSN: 2288-6575; 2288-6796

Purpose: Postsurgical adhesion formation is a significant clinical problem within every surgical specialty. Several adhesion barriers have been developed in the form of solution, membrane or film in an attempt to solve these problems. The purpose of the present study is to compare the efficacy of antiadhesive agents in the prevention of postsurgical adhesion formation in a standardized rat adhesion model. Methods: We examined forty Sprague-Dawley rats, which is a cecal abrasion with partial peritonectomy model. Three treatment groups (Group I: Film-type SurgiwrapⓇ, Group II: Solution-type Guardix-solⓇ, Group III: Membrane-type InterceedⓇ), each consists of 10 rats, and a control group of 10 rats were used by saline. Ten days after surgery, the rats were killed, and the levels of adhesion were graded. Immunohistochemical staining for microvessel density (CD34, MVD) and macrophage (ED1) were performed in adhesion tissue. Results: The peritoneum adhesion mean scores are as follows: control group: 2.2±0.78, Group I: 1.0±1.06, Group II: 0.9±0.99, Group III: 0.6±0.84. All treatment groups showed significantly less peritoneum adhesion (P=0.006), while there was no significant difference in each group. The intraperitoneal organs adhesion mean scores are as follows: control group: 2.8±0.91, Group I: 2.6±1.06, Group II: 1.4±0.84, Group III: 1.0±0.81. Group I had no significant difference about intraperitoneal organs adhesion with control group, but Group II and Group III showed less intraperitoneal organs adhesion. The mean numbers of microvessel density are as follows: control group: 42.5±4.83, Group I: 40.8±6.53, Group II: 30.9±6.15, Group III: 15.60±4.37, from which there was a significant difference between Group II and Group III with control group (P＜0.001). The mean numbers of macrophage are as follows: control group: 223.3±33.12, Group I: 211.25±10.96, Group II: 171.60±23.96, Group III: 147.0± 12.22, from which there was a significant difference between Group II and Group III with control group (P＜0.001). Conclusion: In our animal model, three different types of antiadhesive agents (SurgiwrapⓇ, Guardix-solⓇ, InterceedⓇ) were effective in adhesion prevention, but SurgiwrapⓇ had less antiadhesive effect for intraperitoneal organs adhesion. Membrane-type InterceedⓇ had a better effect for microvessel density (MVD) and macrophage than solution-type Guardix-solⓇ. KCI Citation Count: 17