Rapid fixation of whole organ specimens and attendant problems

Gross specimens are valuable sources in morphology education. In this study, we investigated how the fixation of gross specimens may be accelerated. For this purpose, whole organ specimens from freshly killed rabbits: extremities, kidney, heart, liver, stomach and uterus were fixed in a mercaptoetha...

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Published inActa medica Okayama Vol. 46; no. 2; pp. 75 - 81
Main Authors Durgun-Yücel, B, Dere, F, Yücel, A H, Oguz, O
Format Journal Article
LanguageEnglish
Published Japan 01.04.1992
Subjects
Animals
Formaldehyde
Mercaptoethanol
Organ Preservation
Rabbits
Time Factors
Tissue Fixation - methods
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Abstract Gross specimens are valuable sources in morphology education. In this study, we investigated how the fixation of gross specimens may be accelerated. For this purpose, whole organ specimens from freshly killed rabbits: extremities, kidney, heart, liver, stomach and uterus were fixed in a mercaptoethanol-formaldehyde mixture for 3-3.5h under the following conditions: 1, at room temperature; 2, at gradually increasing temperatures up to 45 degrees C; and 3, at a gradually increasing vacuum ranging from 20 kPa to 40 kPa. The results were compared with those of formaldehyde-fixed controls, and the mercaptoethanol-formaldehyde mixture was found to be useful in shortening the fixation time and providing good fixation. Both heat and vacuum enhanced these phenomena.
AbstractList Gross specimens are valuable sources in morphology education. In this study, we investigated how the fixation of gross specimens may be accelerated. For this purpose, whole organ specimens from freshly killed rabbits: extremities, kidney, heart, liver, stomach and uterus were fixed in a mercaptoethanol-formaldehyde mixture for 3-3.5h under the following conditions: 1, at room temperature; 2, at gradually increasing temperatures up to 45 degrees C; and 3, at a gradually increasing vacuum ranging from 20 kPa to 40 kPa. The results were compared with those of formaldehyde-fixed controls, and the mercaptoethanol-formaldehyde mixture was found to be useful in shortening the fixation time and providing good fixation. Both heat and vacuum enhanced these phenomena.
Author Dere, F
Yücel, A H
Durgun-Yücel, B
Oguz, O
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Snippet Gross specimens are valuable sources in morphology education. In this study, we investigated how the fixation of gross specimens may be accelerated. For this...
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StartPage 75
SubjectTerms Animals
Formaldehyde
Mercaptoethanol
Organ Preservation
Rabbits
Time Factors
Tissue Fixation - methods
Title Rapid fixation of whole organ specimens and attendant problems
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