Trial of isolation of a virus from sera of patients with Kawasaki disease

We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from 24 patients with Kawasaki disease were studied. The specimens were inoculated into HEL cells and Vero cells. The cells were observed for one...

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Published inTokai journal of experimental and clinical medicine Vol. 11; no. 1; p. 19
Main Authors Umehara, K, Kuno-Sakai, H, Iwasaki, H, Takesue, R, Hoshi, N, Furukawa, T, Ikeda, K, Yabe, Y, Kimura, M, Leung, W C
Format Journal Article
LanguageEnglish
Published Japan 01.03.1986
Subjects
Child, Preschool
Humans
Infant
Mucocutaneous Lymph Node Syndrome - microbiology
Viremia - microbiology
Viruses - isolation & purification
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Abstract We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from 24 patients with Kawasaki disease were studied. The specimens were inoculated into HEL cells and Vero cells. The cells were observed for one month, but no cytopathic effect (CPE) occurred. Blind passage was performed, but no degeneration of the second series of cells was observed. Fluorescent antibody indirect methods to determine viral antigens in cells inoculated with patients' specimens was also negative.
AbstractList We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from 24 patients with Kawasaki disease were studied. The specimens were inoculated into HEL cells and Vero cells. The cells were observed for one month, but no cytopathic effect (CPE) occurred. Blind passage was performed, but no degeneration of the second series of cells was observed. Fluorescent antibody indirect methods to determine viral antigens in cells inoculated with patients' specimens was also negative.
Author Ikeda, K
Kuno-Sakai, H
Hoshi, N
Leung, W C
Takesue, R
Furukawa, T
Iwasaki, H
Kimura, M
Yabe, Y
Umehara, K
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Snippet We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from...
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SubjectTerms Child, Preschool
Humans
Infant
Mucocutaneous Lymph Node Syndrome - microbiology
Viremia - microbiology
Viruses - isolation & purification
Title Trial of isolation of a virus from sera of patients with Kawasaki disease
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