Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry

Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We developed a measurement method for OH-PCBs in...

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Bibliographic Details
Published inFukuoka igaku zasshi = Hukuoka acta medica Vol. 102; no. 4; p. 153
Main Authors Tobiishi, Kazuhiro, Todaka, Takashi, Hirakawa, Hironori, Hori, Tsuguhide, Kajiwara, Jumboku, Hirata, Teruaki, Iida, Takao, Uchi, Hiroshi, Furue, Masutaka
Format Journal Article
LanguageEnglish
Published Japan 01.04.2011
Subjects
Chromatography, Liquid
Food Contamination
Humans
Hydroxylation
Oryza - poisoning
Plant Oils - poisoning
Polychlorinated Biphenyls - blood
Polychlorinated Biphenyls - poisoning
Tandem Mass Spectrometry
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Summary:Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We developed a measurement method for OH-PCBs in blood samples by LC/MS/MS. This method is effective at determining the concentrations of PCDDs, PCDFs, Co-PCBs and OH-PCBs from the same sample without special treatment of the sample. The concentration of OH-PCBs in the blood of Yusho patients was examined using this method. The major OH-PCB metabolites were 4-OH-CB187 (54-906 pg/g-wet), 4-OH-CB146 + 3-OH-CB153 (32-527 pg/g-wet), 4-OH-CB109 (ND-229 pg/g-wet) and 4'-OH-CB172 (ND-143 pg/g-wet). The total OH-PCBs ranged from 95 to 1740 pg/g-wet.
ISSN:0016-254X