The application of molecular biological tools to epidemiology of African trypanosomosis

• Published in: Tokai journal of experimental and clinical medicine Vol. 23; no. 6; p. 401
• Main Authors: Eshita, Y, Majiwa, P A, Urakawa, T, Inoue, N, Hirumi, K, Yanagi, T, Yoneda, Y, Hara, T, Higuchi, T, Fukuma, T, Hirumi, H
• Format: Journal Article
• Language: English
• Published: Japan 01.12.1998
• Subjects: Animals; DNA, Protozoan - analysis; Electrophoresis, Gel, Pulsed-Field; Polymerase Chain Reaction - methods; Species Specificity; Trypanosoma - classification; Trypanosoma - genetics; Trypanosoma - isolation & purification; Trypanosoma brucei brucei - classification; Trypanosoma brucei brucei - genetics; Trypanosoma brucei brucei - isolation & purification; Trypanosomiasis, African - diagnosis; Trypanosomiasis, African - epidemiology; Trypanosomiasis, African - parasitology

Difficulties have often been encountered in the field surveys due to a lack of definitive morphological characters, particularly where mixed infections are expected. To address this problem, some molecular biological techniques such as DNA probe hybridization, restriction fragment length polymorphis...