Selective Cytotoxicity of Staphylococcal α-Hemolysin (α-Toxin) against Human Leukocyte Populations

• Published in: Biological and Pharmaceutical Bulletin Vol. 42; no. 6; pp. 982 - 988
• Main Authors: Makoto Tsuijia, Kazuyuki Shioharaa, Yoshinori Takeia, Yoshinori Shinoharaa, Shigeyoshi Nemotoa, Satoshi Yamaguchia, Masanori Kantoa, Saotomo Itoha, Teruaki Okua, Masahiro Miyashitab, Yoshiyuki Seyamab, Masaaki Kuriharac, Tsutomu Tsujia
• Format: Journal Article
• Language: Japanese
• Published: Pharmaceutical Society of Japan 01.06.2019

Staphylococcus aureus produces a variety of exoproteins that interfere with host immune systems. We attempted to purify cytotoxins against human leukocytic cells from the culture supernatant of S. aureus by a combination of ammonium sulfate precipitation, ion-exchange chromatography on a CM-cellulos...