Hyaline Cartilage Regeneration Using Mixed Human Chondrocytes and Transforming Growth Factor-β1- Producing Chondrocytes
The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-β 1 (TGF-β 1 )-producing human chondrocytes (hChon-TGF-β 1 ) and primary human chondrocytes (hChon) ("mixed cells"), compared with either hChon-TGF-β 1 or...
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Published in | Tissue engineering Vol. 11; no. 9-10; pp. 1516 - 1526 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Mary Ann Liebert, Inc
01.09.2005
|
Online Access | Get full text |
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Summary: | The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture
of transforming growth factor-β
1
(TGF-β
1
)-producing human chondrocytes (hChon-TGF-β
1
)
and primary human chondrocytes (hChon) ("mixed cells"), compared with either hChon-TGF-β
1
or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into
the backs of immune-deficient nude mice to test the feasibility of cartilage formation
in vivo
. Both
the mixed cells and the hChon-TGF-β
1
cells alone induced cartilage formation in nude mice, whereas
hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially
induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with
hChon-TGF-β
1
cells with or without mixing additional untransduced hChon cells, and hyaline cartilage
regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and
the quality of tissue generated after implanting were evaluated on the basis of a histological grading
system modified from O'Driscoll
et al
. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed
cells (14.2 ± 0.9) produced significantly better results than hChon-TGF-β
1
(9.0 ± 1.7) or hChon
(8.0 ± 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues
produced after treatment with either mixed cells or hChon-TGF-β
1
cells alone showed hyaline cartilage-
like characteristics. These results suggest that the implantation of mixed cells may be a clinically
efficient method of regenerating hyaline articular cartilage. |
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ISSN: | 1076-3279 |
DOI: | 10.1089/ten.2005.11.1516 |