Effects of Cinnamaldehyde on Salivary Gland Tissue in Xerostomia Model

• Published in: Journal of experimental & biomedical sciences Vol. 26; no. 2; pp. 93 - 100
• Main Authors: Choi, Ja-Hyeong, Lee, Jung-Hwa, Kim, Yeon-Hwa, Hyun, Kyung-Yae, Park, Chung-Mu, Lee, Min-Kyung
• Format: Journal Article
• Language: Korean
• Published: 대한의생명과학회 2020
• Subjects: Submandibular gland; AQP5; Xerostomia; 4-DAMP
• ISSN: 1738-3226; 2288-7415

Xerostomia is a relatively common oral disease that causes various problems such as pain, discomforted, tissue damage, and infection. When the activity of AQPs, which plays an important role in the microbial channel transmembrane activity in tissues, decreases saliva secretion and the oral cavity dryness occurs. In this study, we observed whether there was a change in tissue through the expression level of AQP-5 in the submandibular gland in the 4-DAMP-induced xerostomia model. First, in order to construct a xerostomia model, 4-DAMP (1 mg/kg) and 20% urethane (0.5 mL/kg) were administered intraperitoneal (i.p.) to experimental animals. To observe the changes in the submandibular gland was excised, H&E staining was performed and protein quantitation analysis was performed using the submandibular tissue to observe the changes in AQP5 protein expression involved in changes in saliva secretion. Also, cinnamaldehyde (5, 12.5, 25 and 50 mg/kg) dissolved in 20% DMSO, in distilled water for each concentration, and then orally administered at a dose of 1 mL for biopsy and protein quantitative analysis. As a result, it was observed that the submandibular tissue, a model of xerostomia was wider than the naïve group. And then western blot analysis, the expression level of AQP5 decreased in the 4-DAMP group compared to the naïve group, and the expression increased in the group administered orally with cinnamaldehyde. Therefore, administration of 4-DAMP resulted in histological changes for xerostomia, and cinnamaldehyde would be a material that can be developed by reducing xerostomia.