사향의 크로마토그램 패턴 분석을 통한 품질비교 연구

• Published in: 大韓本草學會誌 Vol. 36; no. 4; pp. 41 - 50
• Main Authors: 조종운, Chong Woon Cho, 가오단, Gao Dan, 강종성, Jong Seong Kang, 정효원, Hyo Won Jung, 박용기, Yong-ki Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.07.2021
• Subjects: Chromatograpy; Identification; Moschus; Moschus moschiferus; Quality comparison; 한의학; Moschus; Identification; Quality comparison; Chromatograpy; Moschus moschiferus
• ISSN: 1229-1765; 2288-7199

Objectives : This study is to check the quality of Moschus products in Korean markets using a chromatographic analysis. Methods : We collected musk products, two genuine products from Russia (A, B), two authentic products from Hong Kong (C) and Korea (D), the Ministry of Food and Drug Safety (MFDS) standard (E), and two fire ants products (E, F). Results : For identification, TLC analysis of 60% ethanol extracts of each product showed that A, B, C, and E have the suitable patterns before color development at 365 nm UV light for the MFDS regulation in Korea. A clear red spot was observed from the E at Rf 0.6 with 365 nm UV light after color development, but this spot was not found in A and B. For the purity test, a distinct violet spot with Rf 0.87 was observed from the A and B in TLC analysis of methanol extracts of each product at white light, however, this spot was not matched with the impurities of E and F. In HPLC-UV pattern analysis, a similar peak pattern was shown in A, B and E, and similar peaks were observed C, although the similarity was weaker than that of A, B, and E. The F and G showed different peak patterns compared with the peak patterns of other samples. Conclusions : Hence, it is considered that the test methods need flexibility in application for identification and purity test depending on the type of sample.