Identification and Cloning of a Fraction 1 Protein of Yersinia pestis that Produces Protective Immune Responses

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 8; pp. 1180 - 1184
• Main Authors: Jong Hyun Kim, Seung Hak Cho, Hyun Chul Jang, Hee Cheul Lee, Young Il Kim, Yeon Ho Kang, Bok Kwon Lee
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.08.2006; 한국미생물·생명공학회
• Subjects: fraction 1 antigen; Y. pestis; 생물학; fraction I antigen; pestis; Y

The capsule that surrounds Yersinia pestis cells is composed of a protein-polysacchride complex; the purified protein component is fraction I (F1) antigen. We report the cloning of the cafl gene and its expression in Escherichia coli using the vector pETl02/D-TOPO and the F1-specific monoclonal anti...