결핵균과 비결핵성항산균 검출에 Real-time PCR의 유용성

• Published in: Tuberculosis and respiratory diseases Vol. 69; no. 4; pp. 250 - 255
• Main Authors: 윤은영, Eun Young Yun, 조수희, Su Hee Cho, 고세일, Se Il Go, 백종하, Jong Ha Baek, 김유은, You Eun Kim, 마정은, Jeong Eun Ma, 이기동, Gi Dong Lee, 조유지, Yu Ji Cho, 정이영, Yi Yeong Jeong, 김호철, Ho Cheol Kim, 이종덕, Jong Deok Lee, 김선주, Sun
• Format: Journal Article
• Language: Korean
• Published: 대한결핵 및 호흡기학회 30.10.2010; 대한결핵및호흡기학회
• Subjects: Atypical; Mycobacteria; Mycobacterium tuberculosis; Polymerase Chain Reaction; Real-Time; 내과학; Mycobacterium tuberculosis; Polymerase Chain Reaction, Real-Time; Mycobacteria, Atypical
• ISSN: 1738-3536; 2005-6184

Background: The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens. Methods: We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using Real-QTM MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTETM MTB II PCR Kit from March 2008 to August 2008. Results: Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens. Conclusion: TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.