rmIL-5로 유도된 호산구의 활성화 및 성장에서 중루의 천식반응 억제효과

• Published in: 大韓本草學會誌 Vol. 20; no. 2; pp. 159 - 169
• Main Authors: 신미경, Mi Kyung Sin, 노성수, Seong Soo Roh, 길기정, Ki Jeong Kil, 이영철, Young Chul Lee, 김진숙, Jin Suk Kim, 서영배, Young Bae Seo
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.06.2005
• Subjects: eosinophil; EPR; extract of Paridis Rhizoma; immunocytochemistry; Lymphokine assay; OVA-induced asthma; 한의학; OVA-induced asthma; lymphokine assay; eosinophil; Immunocytochemistry; EPR (extract of Paridis Rhizoma)
• ISSN: 1229-1765; 2288-7199

Objectives : This study was carried out for the purpose of knowing the effect from anti-arthma action of the abstraction from a extract of Paridis Rhizoma(EPR). In order to know what the effect of controlling an abstraction from Paridis Rhizoma. and about the expression of B cells and Ig E cells, mast cells it was necessary for it to be activated by ovalbumin. Methods : In order to know what the effect was on the organization of cytokine gene expression from The increase and divorce of the B cells and allergic acting by EPR, we found it necessary to examine the BALF. At the same time, as we examined the histamine release by ELISA method, we also examined the effect of EPR. Results : EPR at $100\;{\mu}g/ml$, the highest concentration examined did not have any cytotoxic effects on mLFCs. In FACS analysis, number of granulocyte/lymphocyte, $CD3e^+/CCR3^+,\;CD4^+\;and\;CD23^+/B220^+$ in asthma-induced lung cells were significantly decreased by EPR treatment compared to the control group. In RT-PCR analysis, mRNA expression for CCR3, eotaxin and histamine in asthma-induced lung cells, which was induced by rIL-3 plus rmIL-5 treatments, was significantly decreased by EPR treatment. In ELISA analysis, production levels of IL-4, IL-13 and histamine in asthma-induced lung cells, which were induced by rIL-3 plus rmIL-5 co-treatment, were significantly decreased by EPR treatment. EPR treatments significantly inhibited the proliferation of eosinohils prepared from asthma-induced mouse lung tissues compared to the non-EPR treated control cells. Immunohistochemical analysis revealed that EPR treatment significantly decreased the levels of eosipnphil activation compared to non-treated cells. Conclusion : The present data suggested that Paridis Rhizoma may have an effects on the inhibition of parameters associated with asthma responses in eosinpophils, and thus implicate the possibility for the clinical application of Paridis Rhizoma.