TM3 세포에서 비수리 추출물이 테스토스테론 합성 및 분해에 미치는 영향

• Published in: Yaghag-hoi-ji Vol. 63; no. 3; pp. 137 - 143
• Main Authors: 변재혁(Jae-Hyuk Byun), 유상우(Sang-Woo Yoo), 이강표(Kang-Pyo Lee), 정세영(Se-Young Choung)
• Format: Journal Article
• Language: Korean
• Published: The Pharmaceutical Society Of Korea 30.06.2019; 대한약학회
• Subjects: 약학; TM3 cell; andropause; testosterone; Lespedeza cuneate; hydrogen peroxide
• DOI: 10.17480/psk.2019.63.3.137
• ISSN: 0377-9556

This study focused the effects of Lespedeza cuneata extract (LCE) on testosterone synthesis and degradationin TM3 cells oxidatively stressed by hydrogen peroxide (H2O2). Testosterone level was decreased when the cells weretreated by H2O2, but it was restored to control level by LCE treatment in a dose dependent manner. We examined theexpression of the genes involved in the synthesis or degradation of testosterone to investigate the effect of LEC on thetestosterone metabolic pathway. The gene expression levels of CYP11A1, 17, 20-desmolase, 3β-HSD2 and 17β-HSD3which are involved in testosterone synthesis were decreased by H2O2 and were recovered by LCE treatment. Furthermore,aromatase and 5α-reductase2 which are involved in testosterone degradation were increased by H2O2 and were decreasedto the control level through LCE treatment. In conclusion, LCE restored testosterone level to control level throughincreasing the expression of testosterone synthetase and decreasing the expression of testosterone converting enzyme inoxidatively stressed TM3 cells. We demonstrated that LCE has a protective effect on testosterone reduction followed byandropause state KCI Citation Count: 1