버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 항균활성을 가지는 미생물 Pseudomonas azotoformans HC5

A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene s...

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Published inHangug gynnhaghoi ji Vol. 42; no. 3; pp. 219 - 224
Main Authors 이찬중, 유영미, 한주연, 전창성, 정종천, 문지원, 공원식, 서장선, 한혜수, 차재순, Lee, Chan-Jung, Yoo, Young-Mi, Han, Ju-Yeon, Jhune, Chang-Sung, Cheong, Jong-Chun, Moon, Ji-Won, Gong, Won-Sik, Suh, Jang-Sun, Han, Hye-Su, Cha, Jae-Soon
Format Journal Article
LanguageKorean
Published 한국균학회 01.09.2014
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ISSN0253-651X
2383-5249

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Abstract A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system. P. tolaasii에 의해 발생하는 세균갈색무늬병은 버섯재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균갈색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의 생물검정 실험을 실시하였다. 재배중인 느타리 폐면배지와 양송이 퇴비에서 세균갈색무늬병원균을 강하게 억제하는 길항세균 HC5를 선발하였으며, 생리 생화학적 실험과 유전적 실험결과 HC5균주는 P. azotoformans로 동정되었다. P. azotoformans HC5를 양송이, 팽이버섯, 느타리에 처리한 결과 각각 78%, 73%, 71%의 방제효과를 보였다. 따라서 P. azotoformans HC5가 버섯 세균갈색무늬병 방제를 위해 합성농약을 대체할 수 있는 친환경 방제제가 될 수 있을 것으로 생각된다.
AbstractList P. tolaasii에 의해 발생하는 세균갈색무늬병은 버섯재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균갈색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의생물검정 실험을 실시하였다. 재배중인 느타리 폐면배지와양송이 퇴비에서 세균갈색무늬병원균을 강하게 억제하는길항세균 HC5를 선발하였으며, 생리·생화학적 실험과 유전적 실험결과 HC5균주는 P. azotoformans로 동정되었다. P. azotoformans HC5를 양송이, 팽이버섯, 느타리에 처리한결과 각각 78%, 73%, 71%의 방제효과를 보였다. 따라서 P. azotoformans HC5가 버섯 세균갈색무늬병 방제를 위해 합성농약을 대체할 수 있는 친환경 방제제가 될 수 있을 것으로 생각된다. A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonisticactivity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural,biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but notparasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition invitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus,Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful fordevelopment of a biocontrol system. KCI Citation Count: 6
A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system. P. tolaasii에 의해 발생하는 세균갈색무늬병은 버섯재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균갈색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의 생물검정 실험을 실시하였다. 재배중인 느타리 폐면배지와 양송이 퇴비에서 세균갈색무늬병원균을 강하게 억제하는 길항세균 HC5를 선발하였으며, 생리 생화학적 실험과 유전적 실험결과 HC5균주는 P. azotoformans로 동정되었다. P. azotoformans HC5를 양송이, 팽이버섯, 느타리에 처리한 결과 각각 78%, 73%, 71%의 방제효과를 보였다. 따라서 P. azotoformans HC5가 버섯 세균갈색무늬병 방제를 위해 합성농약을 대체할 수 있는 친환경 방제제가 될 수 있을 것으로 생각된다.
Author 공원식
Suh, Jang-Sun
한혜수
정종천
Han, Ju-Yeon
한주연
Moon, Ji-Won
Gong, Won-Sik
차재순
전창성
문지원
Lee, Chan-Jung
유영미
Cha, Jae-Soon
Yoo, Young-Mi
Han, Hye-Su
서장선
Cheong, Jong-Chun
이찬중
Jhune, Chang-Sung
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Snippet A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The...
P. tolaasii에 의해 발생하는 세균갈색무늬병은 버섯재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균갈색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의생물검정 실험을 실시하였다. 재배중인 느타리 폐면배지와양송이...
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Title 버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 항균활성을 가지는 미생물 Pseudomonas azotoformans HC5
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