Cloning and Characterization of Mannheimia succiniciproducens MBEL55E Phosphoenolpyruvate Carboxykinase (pckA) Gene

A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced a...

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Bibliographic Details
Published inBiotechnology and bioprocess engineering Vol. 7; no. 2; pp. 95 - 99
Main Authors Lee, Sang-Yup, Lee, Pyung-Cheon, Hong, Soon-Ho, Chang, Ho-Nam
Format Journal Article
LanguageKorean
Published 한국생물공학회 01.04.2002
Subjects
생물공학
phosphoenolpyruvate carboxykinase
Mannheimia succiniciproducens MBEL55E
pckA
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ISSN1226-8372
1976-3816

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Summary:A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.
Bibliography:KISTI1.1003/JNL.JAKO200211921118720
G704-000785.2002.7.2.004
ISSN:1226-8372
1976-3816