cDNA microarray 분석을 통한 돼지 지방형질 관련 후보유전자 발굴

• Published in: 농업생명과학연구 Vol. 47; no. 1; pp. 83 - 94
• Main Authors: 이종연, 이경태, 조규호, 김태헌, 김명직, 이현정, 최봉환, 연성흠, 김범수, 박창민, 장길원, Jong-Yeon Lee, Kyung-Tai Lee, Kyu-Ho Cho, Tae-Hun Kim, Myung-Jick Kim, Hyun-Jeong Lee, Bong-Hwan Choi, Seong-Heum Yeon, Bum-Soo Kim, Chang-Min Park, Gul-Won Jang
• Format: Journal Article
• Language: Korean
• Published: 경상국립대학교 농업생명과학연구원 27.02.2013; 농업생명과학연구원
• Subjects: 자연과학일반; 유전자발현 분석칩; Backfat; Real-time PCR; 근내지방; Intramuscular fat contents; 한국재래돼지; cDNA microarray; 등지방; KNP; 실시간 유전자 증폭
• ISSN: 1598-5504; 2383-8272

돼지의 육질과 관련하여 지방침착의 중요도가 높아짐에 따라 지방대사 또는 지방축적과 관련한 후보유전자 발굴 을 위해 등지방 조직 유래의 cDNA microarray를 이용하여 품종별 지방함량관련 조직의 유전자 발현 양상을 분석하 였다. 그 결과 재래돼지의 등지방 조직에서 SCD (stearoyl-CoA desaturase)와 ELOVL6 (elongation of very long chain fatty acid 6)가 높은 발현을 보였고, 간에서 FMO1 (hepatic flavin-containing mono oxygenase)이 높은 발현을 보였으며, 요크셔는 간에서 FGG (fibrinogen gamma polypeptide)와 C3d (com- plement component c3d), 등지방에서 COL3A1 (type Ⅲ collagen alpha 1)이 높은 발현을 보이는 것을 확인하였다. Real-time PCR을 통해 microarray data의 validation과 품종간 유전자 발현량을 비교하여, 위의 유전자들 중 지방함량에 따른 차등발현 유전자로 SCD, ELOVL6 그리고 FGG를 선정하였다. 선정된 유전자 SCD, ELOVL6, FGG는 지방대사에 관여하며 근내지방 함량에도 영향을 미쳐 향후 육질개선에 유용한 후보유전자로 서 활용가능성을 제시하였다. To identify genes involved in fat contents, we analyzed gene expression profiles of porcine backfat tissue using cDNA microarray. The investigated samples were backfat, loin and liver tissues from 12, 24 and 30-week old KNP (Korean Native Pig) and Yorkshire. Micoarray analysis revealed the genes that were differently expressed between KNP and Yorkshire. For KPN, SCD (stearoyl-CoA desaturase) and ELOVL6 (elongation of very long chain fatty acid 6) genes were highly expressed in backfat as FMO1 (hepatic flavin-containing monooxygenase) gene was highly expressed in liver. FGG (fibrinogen gamma polypeptide) and C3d (complement component c3d) genes were highly expressed in liver and COL3A1 (type Ⅲ collagen alpha 1) was highly expressed in backfat for Yorkshire. To validate the highly expressed genes in the microarray data of the gene expressions in two breeds, relative quantitative real-time PCR was used. Consequently, we selected SCD, ELOVL6 and FGG which synthesize monounsaturated fatty acid (18:1) and saturated fatty acid, respectively as the differently expressed genes depending on the fat contents. We found that these fatty acid synthesizing genes, SCD, ELOVL6 and FGG, were positively correlated to the intramuscular fat contents (KNP: 5.3%, Yorkshire: 1.44%). Also, FGG found to be related to lipogenesis in liver. We concluded that the differentially expressed genes in KNP and Yorkshire play important roles in breed specific fat deposition patterns between KNP and Yorkshire.