신규 개인제독키트 , sdk 시제품 ( 가칭 )의 면역독성평가에 관한 고찰 ( evaluation of immunotoxicities of new skin decontamination kit ( sdk ) )

• Published in: Journal of toxicology and public health Vol. 14; no. 2; pp. 205 - 209
• Main Authors: 강병철, 이학모, 제정환, 남정석, 이석만, 양재만, 박재학, 이영순
• Format: Journal Article
• Language: Korean
• Published: 한국독성학회 1998
• Subjects: Delayed contact hypersensitivity; Passive cutaneous anaphylaxis(PCA); Skin decontamination kit(SDK); Max-imization test; Immunotoxicity
• ISSN: 1226-8399

To evaluate immunotoxicity of skin decontamination kit(SDK) newly-developed in Agency for Defense Development(ADD), delayed contact hypersensitivity (maximization) test and passive cutaneous anaphylaxis(PCA) test of SDK were performed and the results were compared with those of M 291. In maximization test, sensitization reaction was induced by id injection (2.5 mg / 0.1 $\textrm{m}{\ell}$/ guinea pig or 2.5 mg+CFA/0.1 $\textrm{m}{\ell}$/guinea pig) and topical application (2.5 mg/$\textrm{m}{\ell}$/guinea pig) with SDK or M291 at an interval of 1 week, and 2 weeks later, challenged by topical application with 25 mg/$\textrm{m}{\ell}$/guinea pig. SDK and M291 did not induce any reactions, showing 0 point of sensitization score and 0% of sensitization rate. In conclusion, it is suggested that SDK and M291 do not induce delayed contact hypersensitivity. In PCA test, rats were administered id with mouse anti-SDK serum and challenged iv with a mixture of antigen SDK and Evan's blue. SDK did not induce blue spots at the injection sites of both high (2.5 mg/mouse) and low (1.25 mg/mouse) dose-induced antisera. In contrast, BSA, positive control produced spots larger than 5 mm in diameter at the injection sites of BSA-induced antiserum up to $2^2$ ~ $2^4$dilution. In conclusion, it is suggested that SDK do not induce IgE production and is not a PCA-reaction inducer.