Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 10; pp. 1446 - 1454
• Main Authors: Huang, Zhen, Ni, Guorong, Zhao, Xiaoyan, Wang, Fei, Qu, Mingren
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 31.10.2021
• Subjects: Bacillus subtilis; characterization; endoglucanase; expression; oligosaccharide; saccharification; endoglucanase; oligosaccharide; expression; Bacillus subtilis; saccharification; characterization

Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp. KSM-330. Results showed that the...