Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection
Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technol...
Saved in:
Published in | Journal of microbiology and biotechnology Vol. 24; no. 3; pp. 363 - 370 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
한국미생물생명공학회
30.03.2014
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control. |
---|---|
AbstractList | Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control. |
Author | Zhi Cheng Liu Zhi Ming Pan Yu Wei Shang Xin An Jiao Yuan Qing Hu Qiu Chun Li Fang Zhe Ren Jin Lin Huang Yang Jiao |
Author_xml | – sequence: 1 fullname: Hu, Yuanqing – sequence: 2 fullname: Huang, Jinlin – sequence: 3 fullname: Li, Qiuchun – sequence: 4 fullname: Shang, Yuwei – sequence: 5 fullname: Ren, Fangzhe – sequence: 6 fullname: Jiao, Yang – sequence: 7 fullname: Liu, Zhicheng – sequence: 8 fullname: Pan, Zhiming – sequence: 9 fullname: Jiao, Xin-An |
BookMark | eNo9kD1PwzAYhC1UJNrCL2DxwhjJX42dsSqlDVTqQGGNEvt1cZvaVZwgIvHjCQJ1utPpuRtugkY-eLhCYyq5SpSSbDR4QmUiFZvdoEmMB0JSylQ6Rt9vEXCwOPf43X2GJPem02Dw3LduDx7vQH_4UId9j9uAcwNDbvsLvvw6NxDjUFiBh_i7tChP574OValbaPABDp13-LFrnN_jdXcq_VC2oFsX_C26tmUd4e5fp-j1ablbrJPNdpUv5pvkOCMy4RYYl5VhOhOUC6gk1yIt9SzTnApiqyzlqTCEGC0JpURQ4KoaeJVZoys-RQ9_q0cXW1d4E-vief6yZYQKqjIx_EAZkwN3f-FicW7cqWz6gjOaCqL4D25uZGc |
ContentType | Journal Article |
DBID | HZB Q5X JDI |
DEWEY | 660.6 579 |
DatabaseName | KISS(한국학술정보) Korean Studies Information Service System (KISS) B-Type KoreaScience |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology Engineering |
DocumentTitleAlternate | Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection |
EISSN | 1738-8872 |
EndPage | 370 |
ExternalDocumentID | JAKO201418942861227 3216408 |
GroupedDBID | --- .UV 29L 53G 5GY 85H 9ZL ACYCR ADBBV AENEX ALMA_UNASSIGNED_HOLDINGS BAWUL DIK DU5 F5P FRP GX1 HZB JDI OK1 P2P Q5X RPM SDH TR2 MZR ZZE |
ID | FETCH-LOGICAL-k507-3fe237bd2c94134eb73c46ac59c3140fb96364d00dc7011041e38bbd289fdcb3 |
ISSN | 1017-7825 |
IngestDate | Fri Dec 22 12:01:20 EST 2023 Wed Jan 24 03:19:52 EST 2024 |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | in vivo-induced antigen technology (IVIAT) foodborne pathogen in vivo-induced gene Campylobacter jejuni |
Language | Korean |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-k507-3fe237bd2c94134eb73c46ac59c3140fb96364d00dc7011041e38bbd289fdcb3 |
Notes | The Korean Society for Applied Microbiology KISTI1.1003/JNL.JAKO201418942861227 |
OpenAccessLink | http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201418942861227&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
PageCount | 8 |
ParticipantIDs | kisti_ndsl_JAKO201418942861227 kiss_primary_3216408 |
PublicationCentury | 2000 |
PublicationDate | 20140330 |
PublicationDateYYYYMMDD | 2014-03-30 |
PublicationDate_xml | – month: 03 year: 2014 text: 20140330 day: 30 |
PublicationDecade | 2010 |
PublicationTitle | Journal of microbiology and biotechnology |
PublicationTitleAlternate | Journal of Microbiology and Biotechnology |
PublicationYear | 2014 |
Publisher | 한국미생물생명공학회 |
Publisher_xml | – name: 한국미생물생명공학회 |
SSID | ssj0061286 ssib053376806 |
Score | 2.017462 |
Snippet | Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes... |
SourceID | kisti kiss |
SourceType | Open Access Repository Publisher |
StartPage | 363 |
SubjectTerms | Campylobacter jejuni foodborne pathogen in vivo-induced antigen technology in vivo-induced gene IVIAT |
Title | Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection |
URI | https://kiss.kstudy.com/ExternalLink/Ar?key=3216408 http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201418942861227&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
Volume | 24 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELW2lZDggKAFUT4qH7itgpI42cTHFS3a3QoQaovKaRU7tkhLncImwCLO_G5m7KybLUV8XKLIcqIo8zTzPJ55JuQpilixJFeBjADBiRZZUAgeBhlLuUaCz2Psd375ajQ5TmYn6clg8KNXtdQ24pn8dm1fyf9YFcbArtgl-w-W9S-FAbgH-8IVLAzXv7LxsUvET83wbfW5DvAYDtzOH5sGNTZ7aXNkmK4lVy_99P2vtggWHkDt6YXr1Dq_WKJECEo4D0_VaWuq4Z7rZHTp_mlXvGV-w2rPqyvKTnDf_JK-n7TW9beF-bgKnXa0S17PKtTv8LVCtuDgTdXK960fPFwlut-1X1TVz11EiW3mC3vuFmMkcBS3r63cWAYuGFzfmo92fdYdFlnP4bLOPbrYzdwhJOuy2lfCnS9CnI0PXuNHRTmHNRgwvTjbIBsswsLQvenByisBH4YlGe5Fu_gOE-35of7bIZgD11_A6gYpf9WjJ0d3yO3OAnTsQHKXDM7qLXLDnTS63CK3erqT2-Q7AIfWmk4N7QOHdsChl8ChTU1XwPHTPXCoBQ6-aQ041AGHOuBQCxzqgXOPHL7YP3o-CbpjOIKzFJPYWsUsE2UsORCeRImMyWRUyJRLBqtzLcCFj5IyDEuZIZlMIsVyAfNzrksp2H2yaWqjHhCaohhhnpcaTAwsPMqLItWccZUWmYp5tEO28UfOL5zOypzFsJYP8x2ya3_s3JSLD_NrrPbwTxMekZuX6HtMNptPrXoCXLIRu9bUPwEULHVN |
link.rule.ids | 230,315,786,790,891 |
linkProvider | Flying Publisher |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Use+of+In+Vivo-Induced+Antigen+Technology+to+Identify+In+Vivo-Expressed+Genes+of+Campylobacter+jejuni+During+Human+Infection&rft.jtitle=Journal+of+microbiology+and+biotechnology&rft.au=Hu%2C+Yuanqing&rft.au=Huang%2C+Jinlin&rft.au=Li%2C+Qiuchun&rft.au=Shang%2C+Yuwei&rft.date=2014-03-30&rft.issn=1017-7825&rft.eissn=1738-8872&rft.volume=24&rft.issue=3&rft.spage=363&rft.epage=370&rft.externalDBID=n%2Fa&rft.externalDocID=JAKO201418942861227 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1017-7825&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1017-7825&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1017-7825&client=summon |