과산화수소 자극으로 활성화된 C6 성상교세포에 대한 맥문동추출물의 조절 효능 연구

• Published in: 大韓本草學會誌 Vol. 35; no. 4; pp. 9 - 16
• Main Authors: 박기호, Ki Ho Park, 강석용, Seok Yong Kang, 정효원, Hyo Won Jung, 박용기, Yong-ki Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.07.2020
• Subjects: Akt; C6 astrocytes; HO-1; Liriope platyphylla; Liriopis tuber; oxidative damage; PKCα; STAT3; Liriope platyphylla; STAT3; PKC{\alpha}; oxidative damage; C6 astrocytes; HO-1; Akt; Liriopis tuber
• ISSN: 1229-1765

Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H 2 O 2 -induced oxidative damage in C6 rat astrocytes. Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 ㎎/㎖ or without for 30 min and then stimulated with H 2 O 2 at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope. Results : LT extract induced cell proliferation in H 2 O 2 -stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H 2 O 2 -stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H 2 O 2 -stimulated C6 cells. Conclusions : LT extract can regulate H 2 O 2 -induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.