Nrf2 활성화(活性化)를 통한 작약(芍藥)의 간보호효과(肝保護效果)

• Published in: 大韓本草學會誌 Vol. 31; no. 1; pp. 33 - 40
• Main Authors: 이수환, Soo Hwan Lee, 정지윤, Ji Yun Jung, 박상미, Sang Mi Park, 제갈경환, Kyung Hwan Jegal, 변성희, Sung Hui Byun, 조일제, Il Je Cho, 김상찬, Sang Chan Kim, 김광중, Kwang Joong Kim, 김영우, Young Woo Kim
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.01.2016
• Subjects: Liver; Nrf2 activation; Oxidative stress; Paeoniae radix; Oxidative stress; Nrf2 activation; Paeoniae radix; Liver
• ISSN: 1229-1765

Objectives : Liver is one of the largest organs in the human, and has a function of detoxification and energy sensing to prevent severe disease. Paeoniae radix has been used to treat a variety of liver diseases such as hepatitis and chronic hepatic failure. Although P. radix has been used as an medicinal herb for a long time, the effects of P. radix on severe oxidative stress and its action mechanism on the liver was not clearly verified. Methods : This study investigated the protective effects of P. radix extract (PRE), and the underlying mechanism of its action in the liver. tert-butyl hydroperoxide (t-BHP) and carbon tetrachlroride (CCl4) were used to induce oxidative stress in the HepG2 hepatocyte cell line and Sprague-Dawley rats, respectively. Results : t-BHP significantly induced cell death and ROS production in HepG2 cell, as indicated by MTT and FACS analysis. However, pretreatment of PRE inhibited a decrease in cell viability and H2O2 production in the HepG2 cells. PRE also blocked the ability of t-BHP to damage in mitochondrial membrane transition. More importantly, PRE induced Nrf2 activation and antioxidant Phase II enzyme, which may have a role in the effects of PRE. In mice, PRE inhibited the liver damage induced by CCl4. Conclusions : PRE inhibited oxidative stress and hepatic damages as mediated with Nrf2 activation. This study unveil, in part, the effect and mechanism of old medicinal herb, P. radix.