Metabolic Changes of Phomopsis longicolla Fermentation and Its Effect on Antimicrobial Activity Against Xanthomonas oryzae

Bacterial blight, an important and potentially destructive bacterial disease in rice caused by Xanthomonas oryzae pv. oryzae (Xoo), has recently developed resistance to the available antibiotics. In this study, mass spectrometry (MS)-based metabolite profiling and multivariate analysis were employed...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 23; no. 2; pp. 177 - 183
Main Authors Choi, Jung Nam, Kim, Jiyoung, Ponnusamy, Kannan, Lim, Chaesung, Kim, Jeong Gu, Muthaiya, Maria John, Lee, Choong Hwan
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 28.02.2013
Subjects
Antimicrobial activity
metabolite profiling
Phomopsis longicolla
principal component analysis
Xanthomonas oryzae
Antimicrobial activity
Phomopsis longicolla
metabolite profiling
principal component analysis
Xanthomonas oryzae
Online AccessGet full text

Cover

More Information
Summary:Bacterial blight, an important and potentially destructive bacterial disease in rice caused by Xanthomonas oryzae pv. oryzae (Xoo), has recently developed resistance to the available antibiotics. In this study, mass spectrometry (MS)-based metabolite profiling and multivariate analysis were employed to investigate the correlation between timedependent metabolite changes and antimicrobial activities against Xoo over the course of Phomopsis longicolla S1B4 fermentation. Metabolites were clearly differentiated based on fermentation time into phase 1 (days 4-8) and phase 2 (days 10-20) in the principal component analysis (PCA) plot. The multivariate statistical analysis showed that the metabolites contributing significantly for phases 1 and 2 were deacetylphomoxanthone B, monodeacetylphomoxanthone B, fusaristatin A, and dicerandrols A, B, and C as identified by liquid chromatography-mass spectrometry (LC-MS), and dimethylglycine, isobutyric acid, pyruvic acid, ribofuranose, galactofuranose, fructose, arabinose, hexitol, myristic acid, and propylstearic acid were identified by gas chromatography-mass spectrometry (GC-MS)-based metabolite profiling. The most significantly different secondary metabolites, especially deacetylphomoxanthone B, monodeacetylphomoxanthone B, and dicerandrol A, B and C, were positively correlated with antibacterial activity against Xoo during fermentation.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO201331056191150
ISSN:1017-7825
1738-8872