Confocal Microscopy

• Published in: Fluorescence Microscopy p. 1
• Main Authors: Naredi‐Rainer, Nikolaus, Prescher, Jens, Hartschuh, Achim, Lamb, Don C
• Format: Book Chapter
• Language: English
• Published: Germany John Wiley & Sons 2017; John Wiley & Sons, Incorporated; Wiley‐VCH Verlag GmbH & Co. KGaA
• Edition: 2nd Edition
• Subjects: Biochemistry, Biology & Biotechnology; Biology & Microbiology; Burst analysis; confocal microscopy; Engineering Principles; fluorescence correlation spectroscopy; General Engineering & Project Administration; multiparameter fluorescence detection; number and brightness; pulsed interleaved excitation; raster image correlation spectroscopy; spinning disk confocal microscope

In chapter 5, we discuss the fundamentals of confocal microscopy. After a brief introduction, we introduce the theory for confocal microscopy and discuss the influence the pinhole has on the axial and lateral resolution of the system. We discuss the optimal configuration of the system depending on t...