마늘 유전자원의 Stemphylium vesicarium에 의한 잎마름병 저항성 평가

Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize c...

Full description

Saved in:
Bibliographic Details
Published inSigmulbyeong yeon'gu Vol. 30; no. 2; pp. 124 - 130
Main Authors 이진주(Jin Ju Lee), 한지원(JiWon Han), 김헌(Hun Kim), 김진철(Jin-Cheol Kim), 최경자(Gyung Ja Choi)
Format Journal Article
LanguageKorean
Published 한국식물병리학회 01.06.2024
Subjects
Online AccessGet full text
ISSN1598-2262
2233-9191
DOI10.5423/RPD.2024.30.2.124

Cover

Loading…
Abstract Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×105 spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. 'Daeseo' and 'Namdo' were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar 'Daeseo'. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight. Stemphylium vesicarium에 의해 발병하는 잎마름병은 마늘재배에 있어 전 세계적으로 가장 중요한 곰팡이 병 중 하나이며, 종자의 품질과 수확량을 감소시킨다. 병 저항성 품종의 재배는 화학 농약의 사용을 감소시키고, 작물 손실을 최소화하는 데 효율적인 방법이다. 본 연구는 잎마름병 저항성 마늘 유전자원을 발굴하기 위하여, 20점의 마늘 유전자원의 잎마름병에 대한 저항성을 조사하기 위하여 실험하였다. 온실에서 재배한 4엽기 유묘의 잎을, 접종 직전에 탈지하지 않은 비흡수성 재질의 솜으로 문지른 후에 멸균한 1/2 농도의 PDB 용액으로 수확한 S. vesicarium KACC 44530 균주의 포자현탁액(3.0×105 spores/ml)을 분무하여 접종하였다. 그리고 접종 3-7일 후에 잎에 발생한 잎마름병의 병반면적률(%)을 조사하였다. 이때 저항성 및 감수성 대조 품종으로 '남도'와 '대서' 마늘이 사용되었다. 실험한 유전자원 중에서, IT245512, IT245528 및 IT244068은 잎마름병에 가장 높은 저항성을 보였으며, 유전자원 IT257134와 IT253043은 잎마름병에 대하여 가장 높은 감수성을 나타내었다. 본 연구에서 선발한 저항성 유전자원은 잎마름병 저항성 육종 시스템에 유용한 자원으로 이용될 수 있을 것이다.
AbstractList Stemphylium vesicarium에 의해 발병하는 잎마름병은 마늘재배에 있어 전 세계적으로 가장 중요한 곰팡이 병 중 하나이며, 종자의 품질과 수확량을 감소시킨다. 병 저항성 품종의 재배는 화학 농약의 사용을 감소시키고, 작물 손실을 최소화하는 데 효율적인 방법이다. 본 연구는 잎마름병 저항성 마늘 유전자원을 발굴하기 위하여, 20점의 마늘 유전자원의 잎마름병에 대한 저항성을 조사하기 위하여 실험하였다. 온실에서 재배한 4엽기 유묘의 잎을, 접종 직전에 탈지하지 않은 비흡수성 재질의 솜으로 문지른 후에 멸균한 1/2 농도의 PDB 용액으로 수확한 S. vesicarium KACC 44530 균주의 포자현탁액(3.0×105 spores/ml)을 분무하여 접종하였다. 그리고 접종 3-7일 후에 잎에 발생한 잎마름병의 병반면적률(%)을 조사하였다. 이때 저항성 및 감수성 대조 품종으로 ‘남도’와 ‘대서’ 마늘이 사용되었다. 실험한 유전자원 중에서, IT245512, IT245528 및 IT244068은잎마름병에 가장 높은 저항성을 보였으며, 유전자원 IT257134 와 IT253043은 잎마름병에 대하여 가장 높은 감수성을 나타내었다. 본 연구에서 선발한 저항성 유전자원은 잎마름병 저항성육종 시스템에 유용한 자원으로 이용될 수 있을 것이다. Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×105 spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. ‘Daeseo’ and ‘Namdo’ were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar ‘Daeseo’. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight. KCI Citation Count: 0
Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×105 spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. 'Daeseo' and 'Namdo' were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar 'Daeseo'. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight. Stemphylium vesicarium에 의해 발병하는 잎마름병은 마늘재배에 있어 전 세계적으로 가장 중요한 곰팡이 병 중 하나이며, 종자의 품질과 수확량을 감소시킨다. 병 저항성 품종의 재배는 화학 농약의 사용을 감소시키고, 작물 손실을 최소화하는 데 효율적인 방법이다. 본 연구는 잎마름병 저항성 마늘 유전자원을 발굴하기 위하여, 20점의 마늘 유전자원의 잎마름병에 대한 저항성을 조사하기 위하여 실험하였다. 온실에서 재배한 4엽기 유묘의 잎을, 접종 직전에 탈지하지 않은 비흡수성 재질의 솜으로 문지른 후에 멸균한 1/2 농도의 PDB 용액으로 수확한 S. vesicarium KACC 44530 균주의 포자현탁액(3.0×105 spores/ml)을 분무하여 접종하였다. 그리고 접종 3-7일 후에 잎에 발생한 잎마름병의 병반면적률(%)을 조사하였다. 이때 저항성 및 감수성 대조 품종으로 '남도'와 '대서' 마늘이 사용되었다. 실험한 유전자원 중에서, IT245512, IT245528 및 IT244068은 잎마름병에 가장 높은 저항성을 보였으며, 유전자원 IT257134와 IT253043은 잎마름병에 대하여 가장 높은 감수성을 나타내었다. 본 연구에서 선발한 저항성 유전자원은 잎마름병 저항성 육종 시스템에 유용한 자원으로 이용될 수 있을 것이다.
Author 이진주(Jin Ju Lee)
최경자(Gyung Ja Choi)
한지원(JiWon Han)
김진철(Jin-Cheol Kim)
김헌(Hun Kim)
Author_xml – sequence: 1
  fullname: 이진주(Jin Ju Lee)
– sequence: 2
  fullname: 한지원(JiWon Han)
– sequence: 3
  fullname: 김헌(Hun Kim)
– sequence: 4
  fullname: 김진철(Jin-Cheol Kim)
– sequence: 5
  fullname: 최경자(Gyung Ja Choi)
BackLink https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003090404$$DAccess content in National Research Foundation of Korea (NRF)
BookMark eNpFkDtLA0EUhQdRMD5-gN02NsKuc---Zsrg-4ERH_UwZmd0SLKR3USws0ihFhoLUfBBKq0ExX9lJv_BjQpW58D57uFwJ8ho2kwVITNAvTBAf35ne9FDioHnUw89wGCElBB93-XAYZSUIOTMRYxwnEznuTmgIY19FlNWIvv91_P-5b1jH3u217HPXfvQtU_3zm5LNY6PTuum3XBOVG6qMiusves6RTq4fXTs89Xw9KXT_7xxbO9scPtmOx_O4Pri6_1sioxpWc_V9J9Okv3lpb2FVXezsrK2UN50axCFLTdJgoTHGhhNQgkRglQHSg-HgmZUc6UlA0giiUzHupiMQRzKiIdYpYmKwJ8kc7-9aaZFrWpEU5ofPWyKWibKO3trAmiMlHEs4NlfuGbylhFpktfFenmjMvwcAg8jSpEx-OfSdmYaKjFSHBdGZqdiq7K4BMAx8jn1vwFESoEH
ContentType Journal Article
DBID DBRKI
TDB
JDI
ACYCR
DEWEY 632
DOI 10.5423/RPD.2024.30.2.124
DatabaseName DBPIA - 디비피아
Nurimedia DBPIA Journals
KoreaScience
Korean Citation Index
DatabaseTitleList

DeliveryMethod fulltext_linktorsrc
Discipline Agriculture
Architecture
DocumentTitleAlternate Evaluation of Garlic Germplasm for Resistance to Leaf Blight Caused by Stemphylium vesicarium
DocumentTitle_FL Evaluation of Garlic Germplasm for Resistance to Leaf Blight Caused by Stemphylium vesicarium
EISSN 2233-9191
EndPage 130
ExternalDocumentID oai_kci_go_kr_ARTI_10720892
JAKO202421956002881
NODE11926390
GroupedDBID 5VS
9ZL
ALMA_UNASSIGNED_HOLDINGS
DBRKI
GROUPED_DOAJ
IPNFZ
JDI
OK1
RIG
RNS
TDB
ACYCR
ID FETCH-LOGICAL-k165t-dd4d97f180d5a1621aebef22621f80f9efa811d6a28f7f7382475a6952c0de613
ISSN 1598-2262
IngestDate Wed Jun 25 03:35:36 EDT 2025
Wed Aug 14 02:11:10 EDT 2024
Thu Feb 06 13:22:08 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 2
Keywords Resistant cultivar
Disease resistance
Screening
Breeding
Garlic germplasm
Language Korean
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-k165t-dd4d97f180d5a1621aebef22621f80f9efa811d6a28f7f7382475a6952c0de613
Notes KISTI1.1003/JNL.JAKO202421956002881
OpenAccessLink http://dx.doi.org/10.5423/RPD.2024.30.2.124
PageCount 7
ParticipantIDs nrf_kci_oai_kci_go_kr_ARTI_10720892
kisti_ndsl_JAKO202421956002881
nurimedia_primary_NODE11926390
PublicationCentury 2000
PublicationDate 2024-06-01
PublicationDateYYYYMMDD 2024-06-01
PublicationDate_xml – month: 06
  year: 2024
  text: 2024-06-01
  day: 01
PublicationDecade 2020
PublicationTitle Sigmulbyeong yeon'gu
PublicationTitleAlternate 식물병연구
PublicationYear 2024
Publisher 한국식물병리학회
Publisher_xml – name: 한국식물병리학회
SSID ssib050738708
ssib036466708
ssib038074770
ssib053377311
ssib013223516
ssib001106841
ssj0000818561
Score 2.2547452
Snippet Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction...
Stemphylium vesicarium에 의해 발병하는 잎마름병은 마늘재배에 있어 전 세계적으로 가장 중요한 곰팡이 병 중 하나이며, 종자의 품질과 수확량을 감소시킨다. 병 저항성 품종의 재배는 화학 농약의 사용을 감소시키고, 작물 손실을 최소화하는 데 효율적인 방법이다. 본 연구는...
SourceID nrf
kisti
nurimedia
SourceType Open Website
Open Access Repository
Publisher
StartPage 124
SubjectTerms 농학
Title 마늘 유전자원의 Stemphylium vesicarium에 의한 잎마름병 저항성 평가
URI https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE11926390
http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO202421956002881&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01
https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003090404
Volume 30
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
ispartofPNX 식물병 연구, 2024, 30(2), , pp.124-130
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Jb9QwFI5KObBICEoRZakigU-jGeKs9jGZSVWK2iLaSr1F2TyMpp2iYQapHFAPPQAHKIeKSpSqJzghgfhXNP0PvJdlEkollotj2c_rs5-_59jPknRXianva0LUVd036jqN_DozBMyrSOOUceoHAvch5xfM2RV9btVYHTszWTm1NBwEjfD5qfdK_oerEAZ8xVuy_8DZUaYQAH7gL7jAYXD_isfEdYhtEcbQw2zCWY24TcKbxFbQAy7T0xCX8DSEO4WnhdRLaJnq8eZaZ7heexYjw_rgTeMtIKyNKIkLrgE51_LsmPtL4baZluQQRyOc1orClTyd3cIQoHAwrkXYDGGcuDZxkKgKkJc67fXhWrAZ4xtI6BLVag-LYZHXx0kbhYVnHo04zZIkr2lBUra7JIGCeVE7IGmeElPJ31GhMdXtEVUvj3EVEp3DoFFzkR-nYQCJ8MhB9kxYsQzkv4c6FW08k-k0u-SdwwOa0Z1ceQw9tYDx6GGrgbVoaEpDbYxSVq18n1h9R2ci5-wHi5hUxRucqAqjQYGzKmg_SmWnIEW5oMazEsXifoJmlChfM3XTtEqTTfikgG6VYhkAvmVpuVhPAQuCttyycN5Z2f9_bNS935oEahzqNh1AY70-gLhzvSG-RAHirILMli9Ll3KVSraz-XFFGutuTEgX7HY_NysTT0gX7cr_sqvSytHnl0ev9-Rk_zA53E4OdpIPO8nHPbkyHeRyOiTvd2SIPd7dl5ODN5j00_bR93dycrh1vPsl2f4mH7999ePr1qS0MuMuN2fr-fsi9S41jUE9ivSIW4IyJTJ8aqrUB4kmsPlUMEXwWPiM0sj0VSYsYWlM1S3DN7mhhkoUAw6-Jo33NnrxdUmGZSzWYksTIQcFJAiDSBgitMLYDNTI53RKmk77zOtFT9e8U1g9Jd2BzvS6YcdDg-_4bW943b4Hau19jyowChhXIZtRZ3tPMnM03sJiy6WgqIGuodz4Uzk3pfPlLLkljQ_6w_g2wOpBMJ0OMnDnX7g_Ab2urCo
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%EB%A7%88%EB%8A%98+%EC%9C%A0%EC%A0%84%EC%9E%90%EC%9B%90%EC%9D%98+Stemphylium+vesicarium%EC%97%90+%EC%9D%98%ED%95%9C+%EC%9E%8E%EB%A7%88%EB%A6%84%EB%B3%91+%EC%A0%80%ED%95%AD%EC%84%B1+%ED%8F%89%EA%B0%80&rft.jtitle=Sigmulbyeong+yeon%27gu&rft.au=%EC%9D%B4%EC%A7%84%EC%A3%BC&rft.au=%ED%95%9C%EC%A7%80%EC%9B%90&rft.au=%EA%B9%80%ED%97%8C&rft.au=%EA%B9%80%EC%A7%84%EC%B2%A0&rft.date=2024-06-01&rft.issn=1598-2262&rft.eissn=2233-9191&rft.volume=30&rft.issue=2&rft.spage=124&rft.epage=130&rft_id=info:doi/10.5423%2FRPD.2024.30.2.124&rft.externalDBID=n%2Fa&rft.externalDocID=JAKO202421956002881
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1598-2262&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1598-2262&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1598-2262&client=summon