감 심지 에탄올 추출물의 항산화 활성 및 신경세포 보호 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 52; no. 1; pp. 60 - 66
• Main Authors: 변의백(Eui-Baek Byun), 김민진(Min-Jin Kim), 김순정(Soon-Jung Kim), 오남순(Nam-Soon Oh), 박상현(Sang-Hyun Park), 김우식(Woo Sik Kim), 송하연(Ha-Yeon Song), 한정무(JeongMoo Han), 김광욱(Kwangwook Kim), 변의홍(Eui-Hong Byun)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.02.2020; Korean Society of Food Science & Technology
• Subjects: Antioxidants; Brain damage; Chemical compounds; Cytotoxicity; Diospyros kaki; Ethanol; Flavonoids; Hippocampus; Hydrogen peroxide; Lipid peroxidation; Lipids; Neuroprotection; Oxidative stress; Peroxidation; Pharmacology; Scavenging; Superoxide dismutase; Toxicity; 식품과학; neuroprotective effects; antioxidant activity; Diospyros kaki Core; neuronal cell
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2020.52.1.60

This study examined the antioxidant activity and neuroprotective effects of ethanol extracts obtained from Diospyros kaki core (DCE). The total polyphenol and flavonoid contents in DCE was 786.47±15.27 and 31.14±0.82 mg/ g, respectively. In addition, DCE exhibited a dose-dependent induction of radical scavenging activity, determined by 1,1diphenyl-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS), ferric reducing antioxidant power (FRAP), and reducing power assays. The viability of HT22 hippocampal cells was examined to investigate the neuroprotective effect of DCE. DCE treatment did not induce cytotoxicity at concentrations below 1,000 µg/mL. Additionally, DCE treatment in the background of H2O2 induce oxidative stress revealed a significant increase in the survival rat, indicated by increased SOD activity and decreased levels of MDA, a lipid peroxidation product. Therefore, the results suggest that DCE can be used as a source of natural antioxidants source and a therapeutic agent for the treatment of brain disorders induced by oxidative stress and neuronal damage.