싸리나무 추출물의 Xanthoangelol 분리 및 HPLC-DAD 밸리데이션

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 52; no. 1; pp. 26 - 30
• Main Authors: 우현심(Hyun Sim Woo), 김영수(Yeong-Su Kim), 오유진(Yu Jin Oh), 조해진(Hae Jin Cho), 송세규(Se-Kyu Song), 김대욱(Dae Wook Kim)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.02.2020; Korean Society of Food Science & Technology
• Subjects: Column chromatography; Columnar structure; Food quality; High performance liquid chromatography; Lespedeza bicolor; Linearity; Liquid chromatography; Quality control; Quantitation; 식품과학; chalcone; xanthoangelol; HPLC-DAD; Lespdeza bicolor; validation
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2020.52.1.26

This study was undertaken to determine the characteristics of xanthoangelol, the major chalcone constituent derived from the extracts of different parts of Lespedeza bicolor. Xanthoangelol was isolated from the root extract using column chromatography and used as a standard for quantitative analysis. The structure of the isolated compound was established based on spectroscopic evidence. The HPLC-DAD method was validated for specificity, linearity, precision, accuracy, limit of detection, and limit of quantitation. The calibration curve of xanthoangelol had significant linearity (R2 >0.9999). Limit of detection and limit of quantitation 0.018 and 0.059 µg/mL, respectively. The relative standard deviation values of precision test, and intra- and inter-day tests were less than 0.22 and 0.40%, respectively. In the recovery test, the accuracy ranged from 98.98-102.78% with RSD values less than 0.13%. The method validation parameters indicate the applicability of the HPLC method for quality control of food or drug formulations containing L. bicolor.