오크라 추출물의 멜라닌 생성저해 및 피부장벽개선 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 50; no. 3; pp. 344 - 348
• Main Author: 김현수(Hyun-Soo Kim)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.06.2018; Korean Society of Food Science & Technology
• Subjects: Abelmoschus esculentus; Barriers; Cosmetics industry; Cytotoxicity; Melanin; Melanoma; Proteins; Skin; Toxicity; Tyrosinase; 식품과학; involucrin; Abelmoschus esculentus; tyrosinase; melanin
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2018.50.3.344

We investigated the application of functional materials by examining a variety of physiological activities of Abelmoschus esculentus extract. A. esculentus extract had a low cytotoxicity against murine melanoma B16F10 cells. At concentrations showing little or no cytotoxicity, A. esculentus extract showed high a DPPH radical scavenging activity (ID50, 5.24 µg/mL), inhibited tyrosinase activity (ID50, 102.12 µg/mL), and decreased melanin content (ID50, 17.85 µg/mL). The treatment of B16F10 cells with A. esculentus extract suppressed the protein expression of tyrosinase in a dosedependent manner. These findings suggest that A. esculentus extract inhibits melanin synthesis in murine melanoma B16F10 cells by suppressing intracellular tyrosinase expression, as well as directly inhibits tyrosinase activity simultaneously. Additionally, A. esculentus extract promotes the expression of involucrin, which is related to skin barrier protection. These results indicate that A. esculentus extract is an appropriate material for improving skin barrier function.