꿀벌 꽃가루 열수 추출물의 큰포식세포 면역활성 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 50; no. 4; pp. 437 - 443
• Main Authors: 김이은(Yi-Eun Kim), 조은지(Eun-Ji Cho), 변의홍(Eui-Hong Byun)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.08.2018; Korean Society of Food Science & Technology
• Subjects: CD80 antigen; Cell activation; Cell surface; Cytokines; Cytotoxicity; IL-1β; Immune system; Immunomodulation; Inflammation; Interleukin 6; Interleukins; Kinases; Macrophages; Major histocompatibility complex; MAP kinase; Nitric oxide; Nitric-oxide synthase; Pollen; Protein kinase; Proteins; Signaling; Toxicity; Tumor necrosis factor-α; 식품과학; mitogen-activated protein kinase; immuno-modulatory activity; cytokine production; macrophage; bee pollen
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2018.50.4.437

Activation of macrophages plays an important role in the host-immune system. In this study, we investigated the functional roles and related signaling mechanism of hot-water extracts of bee pollen (BPW) in RAW 264.7 macrophages. Since BPW did not exert cytotoxicity at concentrations ranging from 62.5 to 250 μg/mL in macrophage cells, a concentration of 250 μg/mL was used as the maximum dose of BPW throughout subsequent experiments. BPW increased inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. Additionally, BPW was found to induce macrophage activation by augmenting the expression of cell surface molecules (cluster of differentiation; CD80/86, and major histocompatibility complex; MHC class I/II) and production of proinflammatory cytokines (tumor necrosis factor-α, interleukin-6, and IL-1β) through mitogen-activated protein kinase and nuclear factor-κB signaling pathways in RAW 264.7 macrophages. Taken together, our results indicate that BPW could potentially be used as an immunomodulatory agent.