1994년부터 1999년까지 대구지방에서 분리된 Shigella sonnei의 분자역학적 분석

• Published in: Journal of bacteriology and virology Vol. 32; no. 1; pp. 23 - 32
• Main Authors: 설성용, Sung Yong Seol, 정영숙, Young Sook Jeong, 유학선, Hak Sun Yu, 이유철, Yoo Chul Lee, 조동택, Dong Taek Cho, 정태훈, Tae Hoon Jung
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 2002; 대한미생물학회
• Subjects: Molecular epidemiology; Shigella sonnei; 생물학; Molecular epidemiology; Shigella sonnei
• ISSN: 1598-2467; 2093-0429

A total of 78 Shigella sonnei strains isolated from 1994 to 1999 in Daegu area were screened by biochemical test, antimicrobial susceptibility test, plasmid profiling, and epidemiologic analysis. Among them, 26 representative strains with different properties were selected and their molecular epidemiological characteristics were studied. Among the 78 strains, two strains did not ferment raffinose and another two strains did not produce colicin. The 26 selected strains were differentiated into 15 plasmid profiles, 11 antimicrobial, 14 enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) pattern, 10 pulsed-field gel electrophoresis (PEGE) pattern types. When all of the typing methods were combined, the strains could be differentiated into 15 types. However, significant correlation among different testing methods was not observed. PEGE analysis indicated that all the strains tested were related despite minor genotypic and phenotypic differences. Our data could not address whether these genetic diversity of strains is due to different circulating strains originated from a number of clone or due to minor genetic variation.