THE CHANGE OF IMMUNE CAPACITY WITH AGING 1. ON THE PERIPHERAL BLOOD LYMPHOCYTE SUBSETS
The change of immune capacity with aging was evaluated by measuring the peripheral blood lymphocyte subsets of 249 normal individuals. Double stained lymphocytes with monoclonal antibodies were analyzed by FACS flowcytometry. It was found that the number of lymphocytes and the proportion of pan T ce...
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| Published in | Japanese Journal of National Medical Services Vol. 47; no. 7; pp. 479 - 485 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japanese Society of National Medical Services
20.07.1993
一般社団法人 国立医療学会 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0021-1699 1884-8729 |
| DOI | 10.11261/iryo1946.47.479 |
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| Abstract | The change of immune capacity with aging was evaluated by measuring the peripheral blood lymphocyte subsets of 249 normal individuals. Double stained lymphocytes with monoclonal antibodies were analyzed by FACS flowcytometry. It was found that the number of lymphocytes and the proportion of pan T cells, suppressor/cytotoxic T cells (CD8+T), cytotoxic T cells and inducer T cells significantly decreased and the proportion of helper T cells significantly increased with aging individuals over 60 years old. The proportion of B cells and suppressor T cells did not significantly change with aging. We also found that the proportion of suppressor-inducer/naive T cells (CD45RA+CD4+T) decreased with aging and helper-inducer/memory T cells (CD45RO+CD4+T) tended to increase until the 50-59 age group and then decrease with aging in the older age groups. The proportion of NK cells, especially the weakest subset of NK cells (CD57+CD16-cells) significantly increased in aged individuals. |
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| AbstractList | The change of immune capacity with aging was evaluated by measuring the peripheral blood lymphocyte subsets of 249 normal individuals. Double stained lymphocytes with monoclonal antibodies were analyzed by FACS flowcytometry. It was found that the number of lymphocytes and the proportion of pan T cells, suppressor/cytotoxic T cells (CD8+T), cytotoxic T cells and inducer T cells significantly decreased and the proportion of helper T cells significantly increased with aging individuals over 60 years old. The proportion of B cells and suppressor T cells did not significantly change with aging. We also found that the proportion of suppressor-inducer/naive T cells (CD45RA+CD4+T) decreased with aging and helper-inducer/memory T cells (CD45RO+CD4+T) tended to increase until the 50-59 age group and then decrease with aging in the older age groups. The proportion of NK cells, especially the weakest subset of NK cells (CD57+CD16-cells) significantly increased in aged individuals. The change of immune capacity with aging was evaluated by measuring the peripheral blood lymphocyte subsets of 249 normal individuals. Double stained lymphocytes with monoclonal antibodies were analyzed by FACS flowcytometry.It was found that the number of lymphocytes and the proportion of pan T cells, suppressor/cytotoxic T cells (CD8+T), cytotoxic T cells and inducer T cells significantly decreased and the proportion of helper T cells significantly increased with aging individuals over 60 years old. The proportion of B cells and suppressor T cells did not significantly change with aging.We also found that the proportion of suppressor-inducer/naive T cells (CD45RA+CD4+T) decreased with aging and helper-inducer/memory T cells (CD45RO+CD4+T) tended to increase until the 50-59 age group and then decrease with aging in the older age groups.The proportion of NK cells, especially the weakest subset of NK cells (CD57+CD16-cells) significantly increased in aged individuals. モノクローナル抗体による二重染色法を行い, FACSフローサイトメトリーによって, 健常人249名の末梢血リンパ球サブセットを測定し, 加齢による免疫能の変化を検討した. その結果, 60歳代以上の高齢者において, 末梢血リンパ球数は加齢とともに有意に減少し, リンパ球サブセットの比率では, T細胞, サプレッサー/サイトトキシックT細胞(CD8+T), サイトトキシックT細胞, インデューサーT細胞の有意な減少, ヘルパーT細胞の有意な増加を認めた. B細胞やサプレッサーT細胞の比率には, 加齢の影響を認めなかった. サプレッサーインデューサー/ナイーブT細胞(CD45RA+CD4+T)の比率は加齢とともに減少を示し, ヘルパーインデューサー/メモリーT細胞(CD45RO+CD4+T)は50歳代までは増加傾向を, 以後加齢とともに逆に低下傾向を示した. NK細胞の坤率は高齢者において有意な増加を示し, 特にNK活性の最も弱いCD57+CD16-NK細胞で顕著であった. |
| Author | HARADA, Yasuko TAKAMOTO, Masahiro HARADA, Susumu ISHIBASHI, Tsuneo KITAHARA, Yoshinari KAJIKI, Akira |
| Author_FL | 原田 進 北原 義也 石橋 凡雄 高本 正祇 原田 泰子 加治 木章 |
| Author_FL_xml | – sequence: 1 fullname: 原田 進 – sequence: 2 fullname: 原田 泰子 – sequence: 3 fullname: 加治 木章 – sequence: 4 fullname: 北原 義也 – sequence: 5 fullname: 高本 正祇 – sequence: 6 fullname: 石橋 凡雄 |
| Author_xml | – sequence: 1 fullname: KITAHARA, Yoshinari organization: Ohmuta National Hospital – sequence: 1 fullname: HARADA, Susumu organization: Department of Neurology, Ohmuta National Hospital – sequence: 1 fullname: KAJIKI, Akira organization: Ohmuta National Hospital – sequence: 1 fullname: HARADA, Yasuko organization: Ohmuta National Hospital – sequence: 1 fullname: ISHIBASHI, Tsuneo organization: Department of Neurology, Ohmuta National Hospital – sequence: 1 fullname: TAKAMOTO, Masahiro organization: Department of Neurology, Ohmuta National Hospital |
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| DocumentTitle_FL | 免疫能の加齢による変化 第１報 末梢血リンパ球サブセットについて |
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| References | 8) 安保徹: NK, LAK細胞の多様性, 臨床免, 22: 1133, 1990 3) Tomer Y et al: Ageing and autoantibodies, Autoimmunity. 1: 141, 1988 9) Walford RL: Immunology and aging, Am J Clin Pathol. 74: 247, 1980 1) Miller RA: Aging and immune function, Int. Rev. Cytol. 124: 187, 1991 2) Schwab R et al: T cell surface determinants and function in aging, Immunology Series. 45: 317, 1989 4) 石橋凡雄他: リンパ球サブセットの正常値及び加齢に伴う変動, 結核, 62: 647, 1987 12) Lewis VM et al: Age, thymic involution, and circulating thymic hormone activity, J. Clin. Endrocrinol. Metabolism. 47: 145, 1978 15) Kristensson K et al: Human CD4+ T cells expressing CD45RA acquire the lymphokine gene expression of CD45RO+ T-helper cells after activation in vitro, Immunology. 76: 103, 1992 11) Resnitzky P et al: Granulopoiesis in aged people: Inverse correlation between bone marrow cellularity and myeloid progenitor cell numbers, Gerontology. 33: 109, 1987 7) Lanier LL et al: Subpopulations of human natural Killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens, J. Immunol. 131: 1789, 1983 17) Hercend T et al: Characteristics and uses of natural killer cells, Immunology Today. 9: 291, 1988 13) Goldstein AL et al: Thymosin and the immunopathology of aging, Federation Proceedings. 33: 2053, 1974 5) Sanders ME et al: Human naive and memory T cells: reinterpretation of helperinducer and suppressor-inducer subsets, Immunology Today, 9: 195, 1988 16) 笠原善仁他: CD4+T 細胞の分化とCD45R分子, 臨床免, 22: 616, 1990 6) Clement LT: Isoforms of the CD45 common leukocyte antigen family: Markers for human T-cell differentiation, J. Clin. Immunol. 12: 1, 1992 14) Pilarski LM et al: Selective expression of CD45 isoforms and of maturation antigens during human thymocyte differentiation: Observations and hypothesis, Immunol. Lett. 21: 187, 1989 18) Lanier LL et al: Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry, Immunol Rev. 74: 143, 1983 10) Kishimoto S et al: Age-related changes in the subsets and functions of human T lymphocytes, J. Immunol. 121: 1773, 1978 |
| References_xml | – reference: 1) Miller RA: Aging and immune function, Int. Rev. Cytol. 124: 187, 1991 – reference: 10) Kishimoto S et al: Age-related changes in the subsets and functions of human T lymphocytes, J. Immunol. 121: 1773, 1978 – reference: 2) Schwab R et al: T cell surface determinants and function in aging, Immunology Series. 45: 317, 1989 – reference: 15) Kristensson K et al: Human CD4+ T cells expressing CD45RA acquire the lymphokine gene expression of CD45RO+ T-helper cells after activation in vitro, Immunology. 76: 103, 1992 – reference: 6) Clement LT: Isoforms of the CD45 common leukocyte antigen family: Markers for human T-cell differentiation, J. Clin. Immunol. 12: 1, 1992 – reference: 4) 石橋凡雄他: リンパ球サブセットの正常値及び加齢に伴う変動, 結核, 62: 647, 1987 – reference: 18) Lanier LL et al: Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry, Immunol Rev. 74: 143, 1983 – reference: 17) Hercend T et al: Characteristics and uses of natural killer cells, Immunology Today. 9: 291, 1988 – reference: 3) Tomer Y et al: Ageing and autoantibodies, Autoimmunity. 1: 141, 1988 – reference: 11) Resnitzky P et al: Granulopoiesis in aged people: Inverse correlation between bone marrow cellularity and myeloid progenitor cell numbers, Gerontology. 33: 109, 1987 – reference: 13) Goldstein AL et al: Thymosin and the immunopathology of aging, Federation Proceedings. 33: 2053, 1974 – reference: 9) Walford RL: Immunology and aging, Am J Clin Pathol. 74: 247, 1980 – reference: 12) Lewis VM et al: Age, thymic involution, and circulating thymic hormone activity, J. Clin. Endrocrinol. Metabolism. 47: 145, 1978 – reference: 14) Pilarski LM et al: Selective expression of CD45 isoforms and of maturation antigens during human thymocyte differentiation: Observations and hypothesis, Immunol. Lett. 21: 187, 1989 – reference: 5) Sanders ME et al: Human naive and memory T cells: reinterpretation of helperinducer and suppressor-inducer subsets, Immunology Today, 9: 195, 1988 – reference: 7) Lanier LL et al: Subpopulations of human natural Killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens, J. Immunol. 131: 1789, 1983 – reference: 8) 安保徹: NK, LAK細胞の多様性, 臨床免, 22: 1133, 1990 – reference: 16) 笠原善仁他: CD4+T 細胞の分化とCD45R分子, 臨床免, 22: 616, 1990 |
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| SubjectTerms | aging flowcytometry peripheral blood lymphocyte subset フローサイトメトリー 加齢 末梢血リンパ球サブセット |
| Subtitle | 1. ON THE PERIPHERAL BLOOD LYMPHOCYTE SUBSETS |
| Title | THE CHANGE OF IMMUNE CAPACITY WITH AGING |
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