EFFECT OF BLEOMYCIN ON THE CHROMOSOMES OF HUMAN LYMPHOCYTES AT VARIOUS CELL PHASES

Bleomycin in various concentrations was added to cultures of peripheral lymphocytes for various periods during culture in order to elucidate quantitatively the cytogenetic effect of the antibiotic on human cells. The antibiotic induced chromatid-type aberrations in G1 cells and the incidence of thes...

Full description

Saved in:
Bibliographic Details
Published inGANN Japanese Journal of Cancer Research Vol. 65; no. 2; pp. 103 - 107
Main Authors SUGIYAMA, Youichi, TAMURA, Hiroaki, SUGAHARA, Tsutomu
Format Journal Article
LanguageEnglish
Published Japan The Japanese Cancer Association 01.01.1974
日本癌学会
Subjects
Bleomycin
Bleomycin - pharmacology
Cells, Cultured
Chromatids
Chromosome
Chromosome Aberrations
Chromosomes - drug effects
DNA - biosynthesis
Human lymphocytes, in vitro
Humans
Lymphocytes - cytology
Lymphocytes - drug effects
Lymphocytes - metabolism
Mitosis
Online AccessGet full text
ISSN0016-450X
DOI10.20772/cancersci1959.65.2_103

Cover

More Information
Summary:Bleomycin in various concentrations was added to cultures of peripheral lymphocytes for various periods during culture in order to elucidate quantitatively the cytogenetic effect of the antibiotic on human cells. The antibiotic induced chromatid-type aberrations in G1 cells and the incidence of these aberrations showed a peak in rather low concentration of the antibiotic. Contrarily, the incidence of acentric fragments and dicentrics induced in G1 cells was increased in linear proportion to the concentration of Bleomycin. The mechanism of the induction of these aberrations remains to be elucidated but the present results imply the possibility for estimating the severity of damage due to Bleomycin in vivo by following the incidence of aberrations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0016-450X
DOI:10.20772/cancersci1959.65.2_103