Application of flow cytometric in situ hybridization assay to Epstein–Barr virus‐associated T/natural killer cell lymphoproliferative diseases
Epstein–Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV‐infected cells using FISH. Using this assay, dual st...
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| Published in | Cancer science Vol. 103; no. 8; pp. 1481 - 1488 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
John Wiley and Sons Inc
01.08.2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1347-9032 1349-7006 1349-7006 |
| DOI | 10.1111/j.1349-7006.2012.02305.x |
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| Abstract | Epstein–Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV‐infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV‐encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV‐associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV‐associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15–67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER‐positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme‐like lymphoma (an EBV‐positive cutaneous T cell lymphoma), EBER‐positive cells were identified as CD3+CD4−CD8− TCRγδ+ T cells. Furthermore, in a 25‐year‐old male patient with systemic EBV‐positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4+CD8− and CD4−CD8+T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV‐infected lymphocytes in EBV‐associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV‐associated diseases. (Cancer Sci, doi: 10.1111/j.1349‐7006.2012.02305.x, 2012) |
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| AbstractList | Epstein-Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell-origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV-infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV-encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV-associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV-associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15-67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER-positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme-like lymphoma (an EBV-positive cutaneous T cell lymphoma), EBER-positive cells were identified as CD3(+) CD4(-) CD8(-) TCRγδ(+) T cells. Furthermore, in a 25-year-old male patient with systemic EBV-positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4(+) CD8(-) and CD4(-) CD8(+) T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV-infected lymphocytes in EBV-associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV-associated diseases. E pstein– B arr virus ( EBV ) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell lymphoproliferative diseases ( T / NK LPD ). Recently, we established a novel assay to identify EBV ‐infected cells using FISH . Using this assay, dual staining with antibodies to both surface antigens and an EBV ‐encoded small RNA ( EBER ) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV ‐associated T / NK LPD to confirm its diagnostic utility. Using FISH , we prospectively analyzed peripheral blood from patients with suspected EBV ‐associated T / NK LPD . The results were compared with those obtained using immunobead sorting followed by quantitative PCR . In all, 26 patients were included study. Using FISH , 0.15–67.0% of peripheral blood lymphocytes were found to be positive for EBER . Dual staining was used to determine EBER ‐positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme‐like lymphoma (an EBV ‐positive cutaneous T cell lymphoma), EBER ‐positive cells were identified as CD 3 + CD 4 − CD 8 − TCR γδ + T cells. Furthermore, in a 25‐year‐old male patient with systemic EBV ‐positive T cell LPD , two lymphocyte lineages were positive for EBER : CD 4 + CD 8 − and CD 4 − CD 8 + T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV ‐infected lymphocytes in EBV ‐associated T / NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV ‐associated diseases. ( Cancer Sci, doi: 10.1111/j.1349‐7006.2012.02305.x, 2012) Epstein-Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell-origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV-infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV-encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV-associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV-associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15-67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER-positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme-like lymphoma (an EBV-positive cutaneous T cell lymphoma), EBER-positive cells were identified as CD3(+) CD4(-) CD8(-) TCRγδ(+) T cells. Furthermore, in a 25-year-old male patient with systemic EBV-positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4(+) CD8(-) and CD4(-) CD8(+) T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV-infected lymphocytes in EBV-associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV-associated diseases.Epstein-Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell-origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV-infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV-encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV-associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV-associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15-67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER-positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme-like lymphoma (an EBV-positive cutaneous T cell lymphoma), EBER-positive cells were identified as CD3(+) CD4(-) CD8(-) TCRγδ(+) T cells. Furthermore, in a 25-year-old male patient with systemic EBV-positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4(+) CD8(-) and CD4(-) CD8(+) T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV-infected lymphocytes in EBV-associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV-associated diseases. Epstein–Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV‐infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV‐encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV‐associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV‐associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15–67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER‐positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme‐like lymphoma (an EBV‐positive cutaneous T cell lymphoma), EBER‐positive cells were identified as CD3+CD4−CD8− TCRγδ+ T cells. Furthermore, in a 25‐year‐old male patient with systemic EBV‐positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4+CD8− and CD4−CD8+T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV‐infected lymphocytes in EBV‐associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV‐associated diseases. (Cancer Sci, doi: 10.1111/j.1349‐7006.2012.02305.x, 2012) Epstein-Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell-origin lymphoma, but also T or natural killer cell lymphoproliferative diseases (T/NK LPD). Recently, we established a novel assay to identify EBV-infected cells using FISH. Using this assay, dual staining with antibodies to both surface antigens and an EBV-encoded small RNA (EBER) probe can be performed. In the present study, we applied this recently developed FISH assay to EBV-associated T/NK LPD to confirm its diagnostic utility. Using FISH, we prospectively analyzed peripheral blood from patients with suspected EBV-associated T/NK LPD. The results were compared with those obtained using immunobead sorting followed by quantitative PCR. In all, 26 patients were included study. Using FISH, 0.15-67.0% of peripheral blood lymphocytes were found to be positive for EBER. Dual staining was used to determine EBER-positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme-like lymphoma (an EBV-positive cutaneous T cell lymphoma), EBER-positive cells were identified as CD3+CD4-CD8- TCR gamma delta + T cells. Furthermore, in a 25-year-old male patient with systemic EBV-positive T cell LPD, two lymphocyte lineages were positive for EBER: CD4+CD8- and CD4-CD8+T cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV-infected lymphocytes in EBV-associated T/NK LPD and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV-associated diseases. (Cancer Sci 2012; 103: 1481-1488) |
| Author | Kawabe, Shinji Ito, Yoshinori Nishiyama, Yukihiro Gotoh, Kensei Kojima, Seiji Iwata, Seiko Kimura, Hiroshi Kinoshita, Tomohiro Matsumoto, Kimikazu |
| AuthorAffiliation | 4 Department of Virology Nagoya University Graduate School of Medicine Nagoya Japan 1 Department of Pediatrics Nagoya University Graduate School of Medicine Nagoya Japan 2 Division of Haematology and Oncology Children's Medical Centre, Nagoya First Red Cross Hospital Nagoya Japan 3 Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan |
| AuthorAffiliation_xml | – name: 2 Division of Haematology and Oncology Children's Medical Centre, Nagoya First Red Cross Hospital Nagoya Japan – name: 4 Department of Virology Nagoya University Graduate School of Medicine Nagoya Japan – name: 1 Department of Pediatrics Nagoya University Graduate School of Medicine Nagoya Japan – name: 3 Department of Hematology and Oncology Nagoya University Graduate School of Medicine Nagoya Japan |
| Author_xml | – sequence: 1 givenname: Shinji surname: Kawabe fullname: Kawabe, Shinji organization: Nagoya University Graduate School of Medicine – sequence: 2 givenname: Yoshinori surname: Ito fullname: Ito, Yoshinori organization: Nagoya University Graduate School of Medicine – sequence: 3 givenname: Kensei surname: Gotoh fullname: Gotoh, Kensei organization: Nagoya University Graduate School of Medicine – sequence: 4 givenname: Seiji surname: Kojima fullname: Kojima, Seiji organization: Nagoya University Graduate School of Medicine – sequence: 5 givenname: Kimikazu surname: Matsumoto fullname: Matsumoto, Kimikazu organization: Children's Medical Centre, Nagoya First Red Cross Hospital – sequence: 6 givenname: Tomohiro surname: Kinoshita fullname: Kinoshita, Tomohiro organization: Nagoya University Graduate School of Medicine – sequence: 7 givenname: Seiko surname: Iwata fullname: Iwata, Seiko organization: Nagoya University Graduate School of Medicine – sequence: 8 givenname: Yukihiro surname: Nishiyama fullname: Nishiyama, Yukihiro organization: Nagoya University Graduate School of Medicine – sequence: 9 givenname: Hiroshi surname: Kimura fullname: Kimura, Hiroshi organization: Nagoya University Graduate School of Medicine |
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| References | 2005; 191 2010; 32 2004; 63 2010; 10 2012; 166 2009; 20 2006; 78 2000; 89 2006; 16 2008; 18 2010; 202 2002; 10 2008; 58 2008 1996; 93 1992; 327 2006 1996; 95 2005; 80 2003; 17 2011; 59 2008; 181 2012; 132 2007; 139 2010; 116 2011; 92 2002; 147 2004; 151 1999; 37 2011; 51 2002; 44 2009; 200 2005; 7 2006; 142 2000; 343 1999; 94 2010; 91 2006; 107 2001; 115 2012; 119 2007; 48 2001; 98 2003; 45 |
| References_xml | – volume: 107 start-page: 862 year: 2006 end-page: 9 article-title: Epstein–Barr virus: the impact of scientific advances on clinical practice publication-title: Blood – volume: 37 start-page: 132 year: 1999 end-page: 6 article-title: Quantitative analysis of Epstein–Barr virus load by using a real‐time PCR assay publication-title: J Clin Microbiol – start-page: 276 year: 2008 end-page: 7 – volume: 63 start-page: 582 year: 2004 end-page: 7 article-title: Clonal expansion of multiphenotypic Epstein–Barr virus‐infected lymphocytes in chronic active Epstein–Barr virus infection publication-title: Med Hypotheses – volume: 98 start-page: 280 year: 2001 end-page: 6 article-title: Clinical and virologic characteristics of chronic active Epstein–Barr virus infection publication-title: Blood – volume: 7 start-page: 495 year: 2005 end-page: 503 article-title: BIOMED‐2 multiplex immunoglobulin/T‐cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics publication-title: J Mol Diagn – volume: 80 start-page: 64 year: 2005 end-page: 9 article-title: Proposed guidelines for diagnosing chronic active Epstein–Barr virus infection publication-title: Am J Hematol – volume: 95 start-page: 116 year: 1996 end-page: 22 article-title: Chronic persistent Epstein–Barr virus infection of natural killer cells and B cells associated with granular lymphocytes expansion publication-title: Br J Haematol – volume: 45 start-page: 1 year: 2003 end-page: 36 article-title: Pathogenic roles for Epstein–Barr virus (EBV) gene products in EBV‐associated proliferative disorders publication-title: Crit Rev Oncol Hematol – volume: 147 start-page: 587 year: 2002 end-page: 91 article-title: Hydroa vacciniforme‐like primary cutaneous CD8‐positive T‐cell lymphoma publication-title: Br J Dermatol – volume: 142 start-page: 587 year: 2006 end-page: 95 article-title: Pathogenic link between hydroa vacciniforme and Epstein–Barr virus‐associated hematologic disorders publication-title: Arch Dermatol – volume: 139 start-page: 532 year: 2007 end-page: 44 article-title: Progress in understanding and managing natural killer‐cell malignancies publication-title: Br J Haematol – volume: 327 start-page: 1710 year: 1992 end-page: 4 article-title: Expression of Epstein–Barr virus‐encoded small RNA (by the EBER‐1 gene) in liver specimens from transplant recipients with post‐transplantation lymphoproliferative disease publication-title: N Engl J Med – volume: 10 start-page: 7 year: 2002 end-page: 14 article-title: Hydroa‐like cutaneous T‐cell lymphoma: a clinicopathologic and molecular genetic study of 16 pediatric cases from Peru publication-title: Appl Immunohistochem Mol Morphol – volume: 151 start-page: 372 year: 2004 end-page: 80 article-title: Epstein–Barr virus‐associated lymphoproliferative lesions presenting as a hydroa vacciniforme‐like eruption: an analysis of six cases publication-title: Br J Dermatol – start-page: 285 year: 2008 end-page: 8 – volume: 200 start-page: 1078 year: 2009 end-page: 87 article-title: Identification of Epstein–Barr virus (EBV)‐infected lymphocyte subtypes by flow cytometric hybridization in EBV‐associated lymphoproliferative diseases publication-title: J Infect Dis – volume: 181 start-page: 5940 year: 2008 end-page: 7 article-title: Identification of CD25+ gamma delta T cells as fetal thymus‐derived naturally occurring IL‐17 producers publication-title: J Immunol – start-page: 278 year: 2008 end-page: 80 – volume: 91 start-page: 42 year: 2010 end-page: 50 article-title: Quantitative analysis of Epstein–Barr virus (EBV)‐related gene expression in patients with chronic active EBV infection publication-title: J Gen Virol – volume: 58 start-page: 209 year: 2008 end-page: 17 article-title: Proposed categorization of pathological states of EBV‐associated T/natural killer‐cell lymphoproliferative disorder (LPD) in children and young adults: overlap with chronic active EBV infection and infantile fulminant EBV T‐LPD publication-title: Pathol Int – volume: 16 start-page: 251 year: 2006 end-page: 61 article-title: Pathogenesis of chronic active Epstein–Barr virus infection: is this an infectious disease, lymphoproliferative disorder, or immunodeficiency? publication-title: Rev Med Virol – volume: 202 start-page: 461 year: 2010 end-page: 9 article-title: Immunologic and virologic analyses in pediatric liver transplant recipients with chronic high Epstein–Barr virus loads publication-title: J Infect Dis – volume: 32 start-page: 832 year: 2010 end-page: 6 article-title: Primary cutaneous Epstein–Barr virus‐associated T‐cell lymphoproliferative disorder: 2 cases with unusual, prolonged clinical course publication-title: Am J Dermatopathol – volume: 18 start-page: 305 year: 2008 end-page: 19 article-title: Measuring Epstein–Barr virus (EBV) load: the significance and application for each EBV‐associated disease publication-title: Rev Med Virol – volume: 119 start-page: 673 year: 2012 end-page: 86 article-title: EBV‐associated T/NK‐cell lymphoproliferative diseases in nonimmunocompromised hosts: prospective analysis of 108 cases publication-title: Blood – volume: 132 start-page: 1401 year: 2012 end-page: 8 article-title: Hydroa vacciniforme is associated with increased numbers of Epstein–Barr virus‐infected γδT cells publication-title: J Invest Dermatol – volume: 166 start-page: 216 year: 2012 end-page: 8 article-title: Phenotypic analysis in a case of hydroa vacciniforme‐like eruptions associated with chronic active Epstein–Barr virus disease of gammadelta T cells publication-title: Br J Dermatol – volume: 59 start-page: 660 year: 2011 end-page: 71 article-title: Clinicopathological analysis of the age‐related differences in patients with Epstein–Barr virus (EBV)‐associated extranasal natural killer (NK)/T‐cell lymphoma with reference to the relationship with aggressive NK cell leukaemia and chronic active EBV infection‐associated lymphoproliferative disorders publication-title: Histopathology – start-page: 306 year: 2008 end-page: 8 – volume: 343 start-page: 481 year: 2000 end-page: 92 article-title: Epstein–Barr virus infection publication-title: N Engl J Med – volume: 17 start-page: 2257 year: 2003 end-page: 317 article-title: Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T‐cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED‐2 Concerted Action BMH4‐CT98–3936 publication-title: Leukemia – volume: 44 start-page: 283 year: 2002 end-page: 94 article-title: Cell type specific infection of Epstein–Barr virus (EBV) in EBV‐associated hemophagocytic lymphohistiocytosis and chronic active EBV infection publication-title: Crit Rev Oncol Hematol – volume: 89 start-page: 1586 year: 2000 end-page: 92 article-title: Malignant lymphoma in southern Taiwan according to the revised European–American classification of lymphoid neoplasms publication-title: Cancer – volume: 116 start-page: 4546 year: 2010 end-page: 9 article-title: Detection of EBV genomes in plasmablasts/plasma cells and non‐B cells in the blood of most patients with EBV lymphoproliferative disorders using Immuno‐FISH publication-title: Blood – volume: 93 start-page: 2272 year: 1996 end-page: 9 article-title: Gamma/delta and other unconventional T lymphocytes: what do they see and what do they do? publication-title: Proc Natl Acad Sci USA – volume: 115 start-page: 105 year: 2001 end-page: 11 article-title: Prospective monitoring of the Epstein–Barr virus DNA by a real‐time quantitative polymerase chain reaction after allogenic stem cell transplantation publication-title: Br J Haematol – start-page: 2655 year: 2006 end-page: 700 – volume: 78 start-page: 770 year: 2006 end-page: 9 article-title: Clonality analysis by sequence variation of the latent membrane protein 1 gene in patients with chronic active Epstein–Barr virus infection publication-title: J Med Virol – volume: 51 start-page: 31 year: 2011 end-page: 7 article-title: Clonal origin of Epstein–Barr virus (EBV)‐infected T/NK‐cell subpopulations in EBV‐positive T/NK‐cell lymphoproliferative disorders of childhood publication-title: J Clin Virol – volume: 92 start-page: 2590 year: 2011 end-page: 5 article-title: Identification of Epstein–Barr virus‐infected CD27+ memory B‐cells in liver or stem cell transplant patients publication-title: J Gen Virol – volume: 48 start-page: 124 year: 2007 end-page: 31 article-title: HLH‐2004: diagnostic and therapeutic guidelines for hemophagocytic lymphohistiocytosis publication-title: Pediatr Blood Cancer – volume: 98 start-page: 3173 year: 2001 end-page: 4 article-title: Mosquito allergy and Epstein–Barr virus‐associated T/natural killer‐cell lymphoproliferative disease publication-title: Blood – volume: 94 start-page: 3094 year: 1999 end-page: 100 article-title: Rapid determination of Epstein–Barr virus‐specific CD8(+) T‐cell frequencies by flow cytometry publication-title: Blood – volume: 10 start-page: 467 year: 2010 end-page: 78 article-title: Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity publication-title: Nat Rev Immunol – volume: 191 start-page: 531 year: 2005 end-page: 9 article-title: Differences between T cell‐type and natural killer cell‐type chronic active Epstein–Barr virus infection publication-title: J Infect Dis – volume: 20 start-page: 1472 year: 2009 end-page: 82 article-title: Epstein–Barr virus‐associated lymphoproliferative disease in non‐immunocompromised hosts: a status report and summary of an international meeting, 8–9 September 2008 publication-title: Ann Oncol |
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| Snippet | Epstein–Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell... Epstein-Barr virus (EBV) infects various types of lymphocytes and is associated with not only B cell-origin lymphoma, but also T or natural killer cell... E pstein– B arr virus ( EBV ) infects various types of lymphocytes and is associated with not only B cell‐origin lymphoma, but also T or natural killer cell... |
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| SubjectTerms | Adolescent Adult Antibodies Cancer CD4 antigen Child Child, Preschool DNA probes Epstein-Barr virus Epstein-Barr Virus Infections - complications Epstein-Barr Virus Infections - immunology Female Flow cytometry Flow Cytometry - methods Fluorescence in situ hybridization Genes, T-Cell Receptor Herpesvirus 4, Human - genetics Herpesvirus 4, Human - immunology Humans Immunoproliferative diseases In Situ Hybridization, Fluorescence - methods Infant Killer Cells, Natural - immunology Lymphocytes B Lymphocytes T Lymphoproliferative Disorders - diagnosis Lymphoproliferative Disorders - pathology Lymphoproliferative Disorders - virology Male Middle Aged Natural killer cells Original Peripheral blood Polymerase chain reaction Prospective Studies RNA RNA probes surface antigens T-cell lymphoma T-cell receptor Young Adult |
| Title | Application of flow cytometric in situ hybridization assay to Epstein–Barr virus‐associated T/natural killer cell lymphoproliferative diseases |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1349-7006.2012.02305.x https://www.ncbi.nlm.nih.gov/pubmed/22497716 https://www.proquest.com/docview/1030079550 https://www.proquest.com/docview/1038617203 https://pubmed.ncbi.nlm.nih.gov/PMC7659396 |
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