Decreasing arsenic accumulation in rice by overexpressing OsNIP1;1 and OsNIP3;3 through disrupting arsenite radial transport in roots

• Published in: The New phytologist Vol. 219; no. 2; pp. 641 - 653
• Main Authors: Sun, Sheng‐Kai, Chen, Yi, Che, Jing, Konishi, Noriyuki, Tang, Zhong, Miller, Anthony J., Ma, Jian Feng, Zhao, Fang‐Jie
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.07.2018
• Subjects: aquaporins; arsenic; Arsenic - metabolism; arsenite; arsenites; Arsenites - metabolism; Biological Transport; Cell Membrane Permeability; corn; food safety; Gene Expression Regulation, Plant; Gene Knockout Techniques; gene overexpression; genes; Nodulin 26‐like Intrinsic Proteins; nutrients; oocytes; Organ Specificity - genetics; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; paddy soils; plant growth; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - metabolism; Plant Shoots - metabolism; Plants, Genetically Modified; plasma membrane; Promoter Regions, Genetic - genetics; radial transport; rice; roots; root‐to‐shoot translocation; Silicon - metabolism; stele; toxicity; transgenic plants; transporters; ubiquitin; Xenopus; xylem; Xylem - metabolism; arsenic; radial transport; rice; root-to-shoot translocation; Nodulin 26-like Intrinsic Proteins; arsenite

Rice is a major dietary source of the toxic metalloid arsenic. Reducing arsenic accumulation in rice grain is important for food safety. We generated transgenic rice overexpressing two aquaporin genes, OsNIP1;1 and OsNIP3;3, under the control of a maize ubiquitin promoter or the rice OsLsi1 promoter...