Functional characterization and expression of endothelin receptors in rat carotid artery: involvement of nitric oxide, a vasodilator prostanoid and the opening of K+ channels in ETB‐induced relaxation

• Published in: British journal of pharmacology Vol. 146; no. 6; pp. 903 - 912
• Main Authors: Tirapelli, Carlos R, Casolari, Debora A, Yogi, Alvaro, Montezano, Augusto C, Tostes, Rita C, Legros, Eurode, D'Orléans‐Juste, Pedro, De Oliveira, Ana M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2005; Nature Publishing
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Carotid Arteries - drug effects; Carotid Arteries - metabolism; Carotid Arteries - physiology; Carotid artery; Endothelin A Receptor Antagonists; Endothelin B Receptor Antagonists; Endothelin-1 - pharmacology; Endothelins - pharmacology; endothelin‐1; Endothelium, Vascular - physiology; Endothelium-Dependent Relaxing Factors - physiology; ETB receptor; Gene Expression - drug effects; Immunohistochemistry; Male; Medical sciences; Nitric Oxide - physiology; Oligopeptides - pharmacology; Peptide Fragments - pharmacology; Peptides, Cyclic - pharmacology; Pharmacology. Drug treatments; Piperidines - pharmacology; Potassium Channels - physiology; Rats; Rats, Wistar; Receptor, Endothelin A - genetics; Receptor, Endothelin A - physiology; Receptor, Endothelin B - genetics; Receptor, Endothelin B - physiology; relaxation; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - genetics; RNA, Messenger - metabolism; Vasodilation - drug effects; Vasodilation - physiology; Vasodilator agent; Rat; Rodentia; relaxation; Ionic channel; Endothelin 1; Inorganic element; Artery; Characterization; ETB receptor; Vertebrata; Mammalia; Blood vessel; Animal; Nitric oxide; ETB endothelin receptor; Prostanoid; Circulatory system; Carotid artery; endothelin-1; Potassium; Endothelin receptor

We aimed to functionally characterize endothelin (ET) receptors in the rat carotid artery. mRNA and protein expressions of both ETA and ETB receptors, evaluated by reverse transcription–polymerase chain reaction (RT–PCR) and Western immunoblotting, were detected in carotid segments. Immunohistochemi...