Oligomerization of the chitin synthase Chs3 is monitored at the Golgi and affects its endocytic recycling

• Published in: Molecular microbiology Vol. 90; no. 2; pp. 252 - 266
• Main Authors: Sacristan, Carlos, Manzano‐Lopez, Javier, Reyes, Abigail, Spang, Anne, Muñiz, Manuel, Roncero, Cesar
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2013
• Subjects: Cell Membrane - metabolism; Chitin; Chitin - biosynthesis; Chitin Synthase - chemistry; Chitin Synthase - metabolism; Endocytosis; Endoplasmic Reticulum - metabolism; Fibers; Golgi Apparatus - metabolism; Membrane Proteins - metabolism; Microbiology; Plasma; Protein Multimerization; Protein Processing, Post-Translational; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Proteins; Quality control; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - metabolism; Yeast

Summary Chs3, the catalytic subunit of chitin synthase III in Saccharomyces cerevisiae, is a complex polytopic membrane protein whose plasma membrane expression is tightly controlled: export from the ER requires interaction with Chs7; exit from the Golgi is dependent on the exomer complex, and preci...