Cell‐surface Vimentin: A mislocalized protein for isolating csVimentin+CD133− novel stem‐like hepatocellular carcinoma cells expressing EMT markers

• Published in: International journal of cancer Vol. 137; no. 2; pp. 491 - 496
• Main Authors: Mitra, Abhisek, Satelli, Arun, Xia, Xueqing, Cutrera, Jeffrey, Mishra, Lopa, Li, Shulin
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 15.07.2015
• Subjects: AC133 Antigen; Animals; Antibodies - immunology; Antibodies - metabolism; Antigens, CD - metabolism; Biomarkers; Biomarkers, Tumor - metabolism; Blotting, Western; Cancer; Carcinoma, Hepatocellular - metabolism; Cell Membrane - metabolism; EMT; Epithelial-Mesenchymal Transition; Genotype & phenotype; Glycoproteins - metabolism; HCC; Humans; Liver cancer; liver cancer stem cells; Liver Neoplasms - metabolism; Medical research; Metastasis; Mice, Inbred NOD; Mice, Knockout; Mice, SCID; Microscopy, Confocal; Neoplasms, Experimental - metabolism; Neoplastic Stem Cells - metabolism; Octamer Transcription Factor-3 - metabolism; Peptides - metabolism; Protein Binding - immunology; Proteins; SOXB1 Transcription Factors - metabolism; Stem cells; Tumor Cells, Cultured; Vimentin - immunology; Vimentin - metabolism; HCC; metastasis; EMT; liver cancer stem cells

Recent advances in cancer stem cell biology have shown that cancer stem‐like cells with epithelial–mesenchymal transition (EMT) phenotypes are more aggressive and cause relapse; however, absence of a specific marker to isolate these EMT stem‐like cells hampers research in this direction. Cell surfac...