Transcript accumulation and utilization of alternate and non-consensus splice sites in rice granule-bound starch synthase are temperature-sensitive and controlled by a single-nucleotide polymorphism

• Published in: Plant molecular biology Vol. 40; no. 4; pp. 719 - 727
• Main Authors: Larkin, P D, Park, W D
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.07.1999
• Subjects: Alternative Splicing - genetics; Amylose - metabolism; Base Sequence; Cultivars; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; High temperature; Molecular Sequence Data; Oryza - enzymology; Oryza - genetics; Oryza sativa; Point Mutation; Polymorphism, Genetic; Rice; RNA Processing, Post-Transcriptional; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Plant - genetics; RNA, Plant - metabolism; Site selection; Starch; Starch Synthase - genetics; Temperature; Transcription, Genetic

Granule-bound starch synthase (GBSS), a product of the waxy gene in rice (Oryza sativa L.), is necessary for the synthesis of amylose in the endosperm. In an extended pedigree of 89 rice cultivars, we have previously shown that all cultivars with more than 18% amylose had the sequence AGGTATA at the...