Coupled Ca2+/H+ transport by cytoplasmic buffers regulates local Ca2+ and H+ ion signaling

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 110; no. 22; pp. E2064 - E2073
• Main Authors: Swietach, Pawel, Youm, Jae-Boum, Saegusa, Noriko, Leem, Chae-Hun, Spitzer, Kenneth W, Vaughan-Jones, Richard D
• Format: Journal Article
• Language: English
• Published: United States National Acad Sciences 28.05.2013; National Academy of Sciences
• Series: PNAS Plus
• Subjects: acetates; adenosine triphosphate; Adenosine Triphosphate - metabolism; Animals; Biological Sciences; buffers; calcium; Calcium Signaling - physiology; cardiomyocytes; carnosine; Cytoplasm - metabolism; Dipeptides - metabolism; fluorescence; Fluorometry; glycolysis; Histidine - metabolism; image analysis; ion exchange; ions; magnesium; Microscopy, Fluorescence; mitochondria; Myocytes, Cardiac - metabolism; PNAS Plus; proteins; Protons; Rats; sarcoplasmic reticulum; pH; dual microperfusion; mobile buffer; calcium; heart

Ca(2+) signaling regulates cell function. This is subject to modulation by H(+) ions that are universal end-products of metabolism. Due to slow diffusion and common buffers, changes in cytoplasmic [Ca(2+)] ([Ca(2+)]i) or [H(+)] ([H(+)]i) can become compartmentalized, leading potentially to complex s...