Methods to study splicing from high-throughput RNA sequencing data

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1126; p. 357
• Main Authors: Alamancos, Gael P, Agirre, Eneritz, Eyras, Eduardo
• Format: Journal Article
• Language: English
• Published: United States 2014
• Subjects: Algorithms; Exons; Gene Expression Profiling; High-Throughput Nucleotide Sequencing - methods; Humans; Molecular Biology - methods; RNA - genetics; RNA Splice Sites - genetics; RNA Splicing - genetics; Sequence Analysis, RNA - methods

The development of novel high-throughput sequencing (HTS) methods for RNA (RNA-Seq) has provided a very powerful mean to study splicing under multiple conditions at unprecedented depth. However, the complexity of the information to be analyzed has turned this into a challenging task. In the last few...