Tissue Distribution of mRNA Expression of Human Cytochrome P450 Isoforms Assessed by High-Sensitivity Real-Time Reverse Transcription PCR

• Published in: YAKUGAKU ZASSHI (Journal of the Pharmaceutical Society of Japan) Vol. 123; no. 5; pp. 369 - 375
• Main Authors: Nishimura, Masuhiro, Yaguti, Hiroshi, Yoshitsugu, Hiroki, Naito, Shinsaku, Satoh, Tetsuo
• Format: Journal Article
• Language: English
• Published: Japan Pharmaceutical Society of Japan 01.05.2003
• Subjects: Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Humans; Isoenzymes - genetics; Isoenzymes - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Sensitivity and Specificity; Tissue Distribution

Pairs of forward and reverse primers and TaqMan probes specific to each human cytochrome P450 isoform were prepared. Analysis of the mRNA level of each CYP isoform in total RNA from pooled specimens of various human or-gans was performed by real-time reverse transcription PCR using an ABI PRISM 7700...