PRODUCTION OF α-FETOPROTEIN BY CULTURED RAT ASCITES HEPATOMA CELLS DETERMINED IN VIVO AS α-FETOPROTEIN-NEGATIVE

Three ascites hepatoma cells of the rat, AH-41B, AH-34, and AH-64B, which had been determined in vivo as α-fetoprotein-negative, were cultivated in vitro in a medium containing adenosine 3', 5'-cyclic monophosphate (cyclic AMP), cyclic dibutyryl-AMP, or theophylline. The concentration of α...

Full description

Saved in:
Bibliographic Details
Published inGANN Japanese Journal of Cancer Research Vol. 68; no. 3; pp. 363 - 367
Main Authors ISAKA, Hidehiko, KOURA, Masatoshi, KOURA, Sumiko, YOSHII, Hirooki, HATTANMARU, Masato, TSUKADA, Yutaka, HIRAI, Hidematsu
Format Journal Article
LanguageEnglish
Published Japan The Japanese Cancer Association 1977
Subjects
alpha-Fetoproteins - biosynthesis
Animals
Ascites hepatoma cells
Carcinoma, Hepatocellular - metabolism
Cell Division - drug effects
Cell Line
Cyclic AMP
Cyclic AMP - pharmacology
Cyclic dibutyryl-AMP
Liver Neoplasms
Neoplasms, Experimental - metabolism
Rats
Theophylline
α-Fetoprotein
Online AccessGet full text

Cover

More Information
Summary:Three ascites hepatoma cells of the rat, AH-41B, AH-34, and AH-64B, which had been determined in vivo as α-fetoprotein-negative, were cultivated in vitro in a medium containing adenosine 3', 5'-cyclic monophosphate (cyclic AMP), cyclic dibutyryl-AMP, or theophylline. The concentration of α-fetoprotein in culture media was measured by the 125I-radioimmunoassay. Results demonstrated that the AH-41B cells produced α-fetoprotein in vitro, the concentration of which being elevated in the media with three substances, while the remaining AH-34 and AH-64B cells did not. A comment was made on the producibility of α-fetoprotein in the so-called α-fetoprotein-negative hepatoma cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0016-450X
DOI:10.20772/cancersci1959.68.3_363