Synthesis of a novel beta-tricalcium phosphate/hydroxyapatite biphasic calcium phosphate containing niobium ions and evaluation of its osteogenic properties

• Published in: Journal of artificial organs Vol. 10; no. 1; pp. 22 - 28
• Main Authors: Tamai, Masato, Isama, Kazuo, Nakaoka, Ryusuke, Tsuchiya, Toshie
• Format: Journal Article
• Language: English
• Published: Japan 01.03.2007
• Subjects: Calcium Phosphates - chemistry; Calcium Phosphates - pharmacology; Humans; Hydroxyapatites - chemical synthesis; Hydroxyapatites - chemistry; Hydroxyapatites - pharmacology; Niobium - pharmacology; Organometallic Compounds - chemical synthesis; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Osteoblasts - drug effects; Osteogenesis - drug effects; Tissue Engineering
• ISSN: 1434-7229; 1619-0904
• DOI: 10.1007/s10047-006-0363-y

To promote the osteogenic properties of osteoblasts, we synthesized a hydroxyapatite (HAp) with beta-tricalcium phosphate (beta-TCP) biphasic calcium phosphate containing Nb ions (NbTCP/HAp). NbTCP/HAp was prepared by annealing precipitates obtained by coprecipitation of an aqueous solution of Ca(NO(3))(2) and a mixture of (NH(4))(2)HPO(4) and aqueous Nb solution. The precipitates can be regarded as a calcium-deficient HAp, the PO(4) sites of which are partly occupied by Nb ions. NbTCP/HAp was successfully synthesized by thermal decomposition of the precipitates. NbTCP/HAp enhanced the calcification of normal human osteoblasts (NHOst), and the amount of calcified tissue increased in proportion to the Nb ion concentration in the NbTCP/HAp. The alkaline phosphatase (ALP) activity of NHOst was also enhanced by NbTCP/HAp. Because Nb ions significantly enhance the ALP activity of NHOst, calcification by NbTCP/HAp is considered to be due to enhancement of ALP activity induced by Nb ions dissolved from NbTCP/HAp. These results indicate that NbTCP/HAp can be an effective bone repair material.