Mechanism of the E2 to E1 transition in Ca2+ pump revealed by crystal structures of gating residue mutants

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 115; no. 50; pp. 12722 - 12727
• Main Authors: Tsunekawa, Naoki, 恒川直樹, Ogawa, Haruo, 小川治夫, Tsueda, Junko, Akiba, Toshihiko, 秋葉俊彦, Toyoshima, Chikashi, 豊島近
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences 11.12.2018
• Subjects: Biological Sciences

Ca2+-ATPase of sarcoplasmic reticulum (SERCA1a) pumps two Ca2+ per ATP hydrolyzed from the cytoplasm and two or three protons in the opposite direction. In the E2 state, after transferring Ca2+ into the lumen of sarcoplasmic reticulum, all of the acidic residues that coordinate Ca2+ are thought to b...