Immortalized mesenchymal stem cells producing conditioned medium in a large scale for therapeutic usage

• Published in: Inflammation and Regeneration Vol. 35; no. 2; pp. 057 - 060
• Main Authors: Ashiba, Keisuke, Terunuma, Atsushi, Terunuma, Hirosh, Takane, Tsubasa, Deng, Xuewen, Yamashita, Yasuhiro, Watanabe, Kazuo
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Society of Inflammation and Regeneration 2015
• Subjects: conditioned medium; dental pulp; immortalization; mesenchymal stem cells; regenerative medicine
• ISSN: 1880-9693; 1880-8190
• DOI: 10.2492/inflammregen.35.057

Stem cells participate in tissue repair not only by generating daughter cells as new cellular components of the tissue being reconstructed but also by secreting biologically active molecules such as cytokines and growth factors to recruit and stimulate other types of cells. Recent studies demonstrated that the conditioned medium of mesenchymal stem cell culture can enhance tissue repair in animal models. In order to produce the conditioned medium of mesenchymal stem cells of constantly high quality in a large scale, we have generated immortalized clones of human mesenchymal stem cells. The immortalized cells displayed similar molecular signatures to those of primary mesenchymal stem cells as indicated by gene expression profiling analysis, and are expected to serve as a stable source of conditioned medium for therapeutic usage. The conditioned medium of the immortalized mesenchymal stem cells is compatible with mass production and mass distribution, and therefore can become available globally at reasonable costs. Since it is also compatible with allogeneic administration, the number of patients benefited by this invention shall be enormous.