Protective effect of Onpi-to (TJ-8117) on the expression of apoptosis in 5/6 nephrectomized rats
The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throug...
Saved in:
| Published in | Nihon Jinzo Gakkai shi Vol. 39; no. 4; pp. 377 - 386 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan
Japanese Society of Nephrology
1997
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-2385 1884-0728 |
| DOI | 10.14842/jpnjnephrol1959.39.377 |
Cover
| Abstract | The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throughout the experiment. All rats were sacrificed at 13 weeks and renal tissues were removed to quantify histopathological and apoptotic parameters in glomeruli. TJ-8117 inhibited proteinuria, matrix index and decrease in the number of total cells in glomeruli of nephrectomized rats. In addition, the increase in apoptotic body and DNA fragmentation was significantly inhibited in the TJ-8117-treated group. Captopril and nicardipine failed to inhibit both parameters. In 400 mg/kg of the TJ-8117 treated group, the number of Bcl-2 positive cells in the glomeruli was elevated compared with the 5/6 nephrectomized control group. In addition, the number of Bax positive cells in the TJ-8117 group was significantly suppressed in a dosedependent manner. These results suggest that TJ-8117 may inhibit apoptosis in the late stage of this model by suppressing matrix accumulation and progression of glomerulosclerosis. The apoptosis-preventing effect may be mediated by decreased expression of Bax in the glomerular cells. |
|---|---|
| AbstractList | The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throughout the experiment. All rats were sacrificed at 13 weeks and renal tissues were removed to quantify histopathological and apoptotic parameters in glomeruli. TJ-8117 inhibited proteinuria, matrix index and decrease in the number of total cells in glomeruli of nephrectomized rats. In addition, the increase in apoptotic body and DNA fragmentation was significantly inhibited in the TJ-8117-treated group. Captopril and nicardipine failed to inhibit both parameters. In 400 mg/kg of the TJ-8117 treated group, the number of Bcl -2 positive cells in the glomeruli was elevated compared with the 5/6 nephrectomized control group. In addition, the number of Bax positive cells in the TJ-8117 group was significantly suppressed in a dose-dependent manner. These results suggest that TJ-8117 may inhibit apoptosis in the late stage of this model by suppressing matrix accumulation and progression of glomerulosclerosis. The apoptosis-preventing effect may be mediated by decreased expression of Bax in the glomerular cells.The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throughout the experiment. All rats were sacrificed at 13 weeks and renal tissues were removed to quantify histopathological and apoptotic parameters in glomeruli. TJ-8117 inhibited proteinuria, matrix index and decrease in the number of total cells in glomeruli of nephrectomized rats. In addition, the increase in apoptotic body and DNA fragmentation was significantly inhibited in the TJ-8117-treated group. Captopril and nicardipine failed to inhibit both parameters. In 400 mg/kg of the TJ-8117 treated group, the number of Bcl -2 positive cells in the glomeruli was elevated compared with the 5/6 nephrectomized control group. In addition, the number of Bax positive cells in the TJ-8117 group was significantly suppressed in a dose-dependent manner. These results suggest that TJ-8117 may inhibit apoptosis in the late stage of this model by suppressing matrix accumulation and progression of glomerulosclerosis. The apoptosis-preventing effect may be mediated by decreased expression of Bax in the glomerular cells. The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throughout the experiment. All rats were sacrificed at 13 weeks and renal tissues were removed to quantify histopathological and apoptotic parameters in glomeruli. TJ-8117 inhibited proteinuria, matrix index and decrease in the number of total cells in glomeruli of nephrectomized rats. In addition, the increase in apoptotic body and DNA fragmentation was significantly inhibited in the TJ-8117-treated group. Captopril and nicardipine failed to inhibit both parameters. In 400 mg/kg of the TJ-8117 treated group, the number of Bcl-2 positive cells in the glomeruli was elevated compared with the 5/6 nephrectomized control group. In addition, the number of Bax positive cells in the TJ-8117 group was significantly suppressed in a dosedependent manner. These results suggest that TJ-8117 may inhibit apoptosis in the late stage of this model by suppressing matrix accumulation and progression of glomerulosclerosis. The apoptosis-preventing effect may be mediated by decreased expression of Bax in the glomerular cells. The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril (50 mg/kg/day) and nicardipine (50 mg/kg/day) were administered as drinking water from the 56th day after renal ablation, and continued throughout the experiment. All rats were sacrificed at 13 weeks and renal tissues were removed to quantify histopathological and apoptotic parameters in glomeruli. TJ-8117 inhibited proteinuria, matrix index and decrease in the number of total cells in glomeruli of nephrectomized rats. In addition, the increase in apoptotic body and DNA fragmentation was significantly inhibited in the TJ-8117-treated group. Captopril and nicardipine failed to inhibit both parameters. In 400 mg/kg of the TJ-8117 treated group, the number of Bcl -2 positive cells in the glomeruli was elevated compared with the 5/6 nephrectomized control group. In addition, the number of Bax positive cells in the TJ-8117 group was significantly suppressed in a dose-dependent manner. These results suggest that TJ-8117 may inhibit apoptosis in the late stage of this model by suppressing matrix accumulation and progression of glomerulosclerosis. The apoptosis-preventing effect may be mediated by decreased expression of Bax in the glomerular cells. |
| Author | SHINDO, Shoichiro KUROGI, Akiko HATTORI, Tomohisa FUJITSUKA, Naoki |
| Author_xml | – sequence: 1 fullname: HATTORI, Tomohisa organization: TSUMURA and Co. Central Laboratories, New Drug Research Department 1 – sequence: 1 fullname: FUJITSUKA, Naoki organization: TSUMURA and Co. Central Laboratories, New Drug Research Department 1 – sequence: 1 fullname: SHINDO, Shoichiro organization: TSUMURA and Co. Central Laboratories, New Drug Research Department 1 – sequence: 1 fullname: KUROGI, Akiko organization: TSUMURA and Co. Central Laboratories, New Drug Research Department 1 |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9198359$$D View this record in MEDLINE/PubMed |
| BookMark | eNpdkE9P3DAQxS0Eosufj4DqU1UOAU9sx_axQoUWkOAA5-Akk65XWTu1vRXw6bFgxaHS6M2M3k9Pozkguz54JOQrsDMQWtTnq9mvPM7LGCYw0pzxUkrtkAVoLSqmar1LFoxrWdVFvpDjlFzHQCvGpRL7ZN-A0VyaBXm6jyFjn90_pDiOZaJhpHd-dlUO9PvDdaUB1CkNnuZlQZ7niCWsrAWzc5hzSC5R56k8b-j7TSUjrN0rDjTanI7I3minhMfbfkgeL38-XPyqbu-ufl_8uK1WtWhy1RkGarBMcNWbQajR8AZ6YAi1qDvW1WB1UzdSjAMMArQZpUJoJLBuYCgtPyTfPnLnGP5uMOV27VKP02Q9hk1qlWFKM-AFPNmCm26NQztHt7bxpd2-pPg3H_4qZfsHP30bs-snbP_7fctNK7ai1CfVL21s0fM36aCCzQ |
| ContentType | Journal Article |
| Copyright | Japanese Society of Nephrology |
| Copyright_xml | – notice: Japanese Society of Nephrology |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.14842/jpnjnephrol1959.39.377 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| EISSN | 1884-0728 |
| EndPage | 386 |
| ExternalDocumentID | 9198359 article_jpnjnephrol1959_39_4_39_4_377_article_char_en |
| Genre | English Abstract Journal Article |
| GroupedDBID | ALMA_UNASSIGNED_HOLDINGS JSF KQ8 OK1 RJT CGR CUY CVF ECM EIF NPM 7X8 |
| ID | FETCH-LOGICAL-j246t-b9017da0437c9d47f9361c10e1242b0b21a862654fd1d4189f57e16510bd0e5a3 |
| ISSN | 0385-2385 |
| IngestDate | Fri Jul 11 13:26:40 EDT 2025 Wed Feb 19 01:38:43 EST 2025 Wed Sep 03 06:06:30 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 4 |
| Language | Japanese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-j246t-b9017da0437c9d47f9361c10e1242b0b21a862654fd1d4189f57e16510bd0e5a3 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/jpnjnephrol1959/39/4/39_4_377/_article/-char/en |
| PMID | 9198359 |
| PQID | 79078013 |
| PQPubID | 23479 |
| PageCount | 10 |
| ParticipantIDs | proquest_miscellaneous_79078013 pubmed_primary_9198359 jstage_primary_article_jpnjnephrol1959_39_4_39_4_377_article_char_en |
| PublicationCentury | 1900 |
| PublicationDate | 1997-00-00 |
| PublicationDateYYYYMMDD | 1997-01-01 |
| PublicationDate_xml | – year: 1997 text: 1997-00-00 |
| PublicationDecade | 1990 |
| PublicationPlace | Japan |
| PublicationPlace_xml | – name: Japan |
| PublicationTitle | Nihon Jinzo Gakkai shi |
| PublicationTitleAlternate | Jpn J Nephrol |
| PublicationYear | 1997 |
| Publisher | Japanese Society of Nephrology |
| Publisher_xml | – name: Japanese Society of Nephrology |
| References | 15. Dworkin LD, Feiner HD, Parker M, Tolbert E. Effects of nifedipine and enalapril on glomerular structure and function in uninephrectomized SHR. Kidney Int 1991 ; 39 :1112-1117. 14. 塩之入洋,木原 実,成瀬雅彦,他.アンジオテンシン変換酵素阻害薬.腎と透析(増刊号)1995;39:563-572. 4. Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, Yamanaka N. Apoptosis in progressive crescentic glomerulonenhritis. Lab Invest 1996 ; 74 : 941-951. 16. Dworkin LD, Benstein JA, Feiner HD, Parker M, Tolbert E. Calcium antagonist and converting enzyme inhibitors reduce renal injury by different mechanisms. Kidney Int 1993 ; 43 808-814. 2. Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugisaki Y, Yamanaka N. Apoptosis in the repair process of experimental proliferative glomerulonephritis. Kidney Int 1995 ; 47 : 114-121. 1. Baker AJ, Mooney A, Hughes J, Lombardi D, Johnson RJ, Savill J. Mesangial cell apoptosis : The major mechanism for resolution of glomerular hypercellularity in experimental mesangial proliferative nephritis. J Clin Invest 1994 ; 94 : 2105-2116. 13. 吉村吾志夫,宇田 普.メサンギウム増殖性腎炎におけるアポトーシス関連分子発現とその意義医学の歩み1996;178:761-764. 10. 藤塚直樹,服部智久,進藤省一郎.5/6腎摘出ラットにおける糸球体の増殖変化に対するTJ-8117の作用とその活1生成分について.日腎会誌1995;37:162(Suppl). 11. 槇野博史,杉山 斉,柏原直樹.糸球体硬化進展過程におけるアポトーシス.医学の歩み1996;178:770-775. 8. Mistuma T, Yokozawa T, Oura H, Terasawa K. Rhubarb therapy in patients with chronic renal failure (Part 2) Japan J Nephrology 1987 ; 34 : 73-85. 9. moue M, Suzuki R, Koide T, Sakaguchi N, Ogihara Y, Yabu Y. Antioxidant, gallic acid, induces apoptosis in HL 60 RG cells. Biochem and Biophys Res Commun 1994; 204: 898-904. 5. 服部智久,藤塚直樹,黒木亮子,進藤省一郎.慢性腎不全モデルにおける糸球体増殖因子とアポトーシス発現の変動.日本腎臓学会誌1996;38:Suppl.85. 6. 服部智久,進藤省一郎,久田孝光,藤塚直樹,日比野智子,寺園芳樹,丸野政雄.抗体で惹起された糸球体メサンギウム障害に対するTJ-8117の効果日薬理誌1995;105:63-75. 7. 服部智久,藤塚直樹,黒木亮子,進藤省一郎.5/6腎摘モデルにおけるTGFβ1産生に対する温脾湯(TJ-8117)の効果.日腎会誌1996;38:475-483. 3. Sugiyama H, Kashihara N, Makino H, Yamasaki Y, Ota Z. Apoptosis in glomerular sclerosis. Kidney Int 1996 ; 49 :103-111. 12. 寺田典生.アポトーシス関連遺伝子の腎内分布とその発現.医学の歩み1996;178:723-727. |
| References_xml | – reference: 1. Baker AJ, Mooney A, Hughes J, Lombardi D, Johnson RJ, Savill J. Mesangial cell apoptosis : The major mechanism for resolution of glomerular hypercellularity in experimental mesangial proliferative nephritis. J Clin Invest 1994 ; 94 : 2105-2116. – reference: 3. Sugiyama H, Kashihara N, Makino H, Yamasaki Y, Ota Z. Apoptosis in glomerular sclerosis. Kidney Int 1996 ; 49 :103-111. – reference: 8. Mistuma T, Yokozawa T, Oura H, Terasawa K. Rhubarb therapy in patients with chronic renal failure (Part 2) Japan J Nephrology 1987 ; 34 : 73-85. – reference: 6. 服部智久,進藤省一郎,久田孝光,藤塚直樹,日比野智子,寺園芳樹,丸野政雄.抗体で惹起された糸球体メサンギウム障害に対するTJ-8117の効果日薬理誌1995;105:63-75. – reference: 9. moue M, Suzuki R, Koide T, Sakaguchi N, Ogihara Y, Yabu Y. Antioxidant, gallic acid, induces apoptosis in HL 60 RG cells. Biochem and Biophys Res Commun 1994; 204: 898-904. – reference: 4. Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, Yamanaka N. Apoptosis in progressive crescentic glomerulonenhritis. Lab Invest 1996 ; 74 : 941-951. – reference: 10. 藤塚直樹,服部智久,進藤省一郎.5/6腎摘出ラットにおける糸球体の増殖変化に対するTJ-8117の作用とその活1生成分について.日腎会誌1995;37:162(Suppl). – reference: 12. 寺田典生.アポトーシス関連遺伝子の腎内分布とその発現.医学の歩み1996;178:723-727. – reference: 14. 塩之入洋,木原 実,成瀬雅彦,他.アンジオテンシン変換酵素阻害薬.腎と透析(増刊号)1995;39:563-572. – reference: 15. Dworkin LD, Feiner HD, Parker M, Tolbert E. Effects of nifedipine and enalapril on glomerular structure and function in uninephrectomized SHR. Kidney Int 1991 ; 39 :1112-1117. – reference: 11. 槇野博史,杉山 斉,柏原直樹.糸球体硬化進展過程におけるアポトーシス.医学の歩み1996;178:770-775. – reference: 13. 吉村吾志夫,宇田 普.メサンギウム増殖性腎炎におけるアポトーシス関連分子発現とその意義医学の歩み1996;178:761-764. – reference: 16. Dworkin LD, Benstein JA, Feiner HD, Parker M, Tolbert E. Calcium antagonist and converting enzyme inhibitors reduce renal injury by different mechanisms. Kidney Int 1993 ; 43 808-814. – reference: 5. 服部智久,藤塚直樹,黒木亮子,進藤省一郎.慢性腎不全モデルにおける糸球体増殖因子とアポトーシス発現の変動.日本腎臓学会誌1996;38:Suppl.85. – reference: 7. 服部智久,藤塚直樹,黒木亮子,進藤省一郎.5/6腎摘モデルにおけるTGFβ1産生に対する温脾湯(TJ-8117)の効果.日腎会誌1996;38:475-483. – reference: 2. Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugisaki Y, Yamanaka N. Apoptosis in the repair process of experimental proliferative glomerulonephritis. Kidney Int 1995 ; 47 : 114-121. |
| SSID | ssib018703574 ssib004261733 ssib058493074 ssib000833381 ssib002004354 ssib005879731 ssib039893136 ssib008531636 ssib038076193 ssj0000696431 ssib000940323 ssib002822005 ssib038586121 ssib047305546 |
| Score | 1.4844232 |
| Snippet | The present study was designed to clarify the effects of TJ-8117 on apoptosis in the glomeruli of 5/6 nephrectomized rats. TJ-8117 (400 mg/kg/day), captopril... |
| SourceID | proquest pubmed jstage |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 377 |
| SubjectTerms | 5/6 nephrectomy, rats, TJ-8117, apoptosis, Bcl-2, Bax Animals Apoptosis - drug effects Depression, Chemical DNA Fragmentation - drug effects Dose-Response Relationship, Drug Drugs, Chinese Herbal - pharmacology Kidney Glomerulus - cytology Male Nephrectomy Rats Rats, Wistar |
| Title | Protective effect of Onpi-to (TJ-8117) on the expression of apoptosis in 5/6 nephrectomized rats |
| URI | https://www.jstage.jst.go.jp/article/jpnjnephrol1959/39/4/39_4_377/_article/-char/en https://www.ncbi.nlm.nih.gov/pubmed/9198359 https://www.proquest.com/docview/79078013 |
| Volume | 39 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | The Japanese Journal of Nephrology, 1997, Vol.39(4), pp.377-386 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFLZgCGkXBIKJ8tMHDqAqWxI7cXxEjDE6MSHRSbsFp3HWpCyJ1vTSv573YjdJyyYBl6hxklZ978vz9-znz4S8m2VS6DD0nSTUrsNVCq9UppWTaZnhrtNJlODQwLfz8PSCTy6Dy76IvV1d0iSHs_Wt60r-x6vQBn7FVbL_4NnuS6EBPoN_4QgehuNf-fi7EVnA4h9Tl4HUryrr3AFGCdRxOnFwhSdm_mZOAAX9TeFryxJVXdVNhZIkeTlG9YdwXGr07qyprvM1cFHAx3LIX8_zOTw6yct1Nf6iFguVj5fzvA9jDWqObNVen6yKvFmuFmpr3udsdQMxd2ss9cc8L9OqH41N7do8MYhVLAoc6P2DYWA1KkUWQHwQJZnZueWP6M0jjnKwRV0W7b-tfqH0zSE4fucJcEN93TpVehI4pOx7s67G0F65Tx74QnhY7Xn89WzIPCE5HzBhyV02ZII4RTpUmgMitSPs74kBUw4igZuAdefAgoDpdkzPg7DIgp5po8w_pK6sPw8i1HLrziXwSK9_ngtUZuszd6CNkm1kjAzpQGG1dn_IjStsNSMa9eh2k-6Th9ZKwL8KyEau9N2JVku4po_JI5sp0Y8G9k_IvUI9JT97yFMDeVpl1EKevreA_wAtFOBOe7jjbR3caV7S4Cik22CnCPZn5OLk8_TTqWM3CXEKn4eNkwChFalCia6ZTLnIJAu9medqIK5-4ia-pzBpD3iWein3IghBQnshdEVJ6upAsQOyV1alfk5oELX5ByQYPOOhmyiRRVHKdJIKpqOUj8ixMVJcGyWY2L758Y55YyZjbg9CdHfhOkoIWyPydmPiGGI5TtCpUlerZSwkEHbIyUbkwFi--yHrphd3XXhJ9o2uMo4NviJ7zc1Kvwa23CRvWtz_BnIqp7s |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Protective+effect+of+onpi-to+%28TJ-8117%29+on+the+expression+of+apoptosis+in+5%2F6+nephrectomized+rats&rft.jtitle=Nihon+Jinzo+Gakkai+shi&rft.au=Hattori%2C+T&rft.au=Fujitsuka%2C+N&rft.au=Kurogi%2C+A&rft.au=Shindo%2C+S&rft.date=1997&rft.issn=0385-2385&rft.volume=39&rft.issue=4&rft.spage=377&rft_id=info:doi/10.14842%2Fjpnjnephrol1959.39.377&rft_id=info%3Apmid%2F9198359&rft.externalDocID=9198359 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0385-2385&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0385-2385&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0385-2385&client=summon |