Effects of Intravenous 2-chloroadenosine on Endotoxin-Induced Acute Lung Injury

• Published in: Nihon Kyōbu Shikkan Gakkai zasshi Vol. 34; no. 6; pp. 665 - 670
• Main Authors: Ishizaka, Akitoshi, Sakamaki, Fumio, Sayama, Kouichi, Urano, Tetsuya, Nakamura, Hidetoshi, Terashima, Takeshi, Waki, Yasuhiro, Soejima, Kenzo, Tasaka, Sadatomo, Kanazawa, Minoru
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Respiratory Society 01.06.1996
• Subjects: 2-chloroadenosine; 2-Chloroadenosine - administration & dosage; 2-Chloroadenosine - pharmacology; Acute lung injury; Animals; Endotoxin; Endotoxins - adverse effects; Female; Guinea Pigs; Infusions, Intravenous; Pulmonary edema; Respiratory Distress Syndrome, Adult - etiology; Respiratory Distress Syndrome, Adult - prevention & control
• ISSN: 0301-1542; 1883-471X
• DOI: 10.11389/jjrs1963.34.665

To assess the effects of 2-chloroadenosine (2CA) on acute lung injury caused by endotoxin (lipopolysaccharide), guinea pigs were given 2CA intravenously. Three groups were used: saline control, endotoxin control and 2CA+ endotoxin. In the endotoxin and 2CA+ endotoxin groups, neutrophils accumulated in bronchoalveolar lavage fluid and in lung tissue. Howevre, neutrophil accumulation did not differ significantly between the endotoxin and the 2CA+ endotoxin groups. The number of macrophages in bronchoalveolar lavage fluid was signficantly higher in the endotoxin group than in the saline control group, but the difference between the saline control and the 2CA+ endotoxin groups was not significant. The lung wet-dry weight ratio and 125I-albumin lung tissue-plasma ratio, which were used to measure acute lung injury, were significantly higher in the endotoxin group than in the 2CA+ endotoxin and the saline control groups. However, these ratios did not differ between the 2CA+ endotoxin and the saline control groups. These results suggest that 2CA attenuated endotoxin induced acute lung injury in guinea pigs.